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PMID:8753653
| Citation |
Reymond-Cotton, P, Fraissinet-Tachet, L and Fèvre, M (1996) Expression of the Sclerotinia sclerotiorum polygalacturonase pg1 gene: possible involvement of CREA in glucose catabolite repression. Curr. Genet. 30:240-5 |
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| Abstract |
Northern-blot analysis of RNA isolated from Sclerotinia sclerotiorum grown on either glucose or polygalacturonate as the sole carbon source showed that pg1, encoding a neutral polygalacturonase, was not expressed during growth in both media. In contrast, transcripts of this gene were detected during infection of sunflower germlings. Analysis of the promoter sequence revealed a number of cis-acting sequences known to regulate the expression of many fungal promoters. Protein-DNA-binding experiments showed that proteins extracted from mycelia grown on polygalacturonate or glucose interacted with different regions of the promoter. The GST-CREA fusion protein, containing the two zinc fingers of the Aspergillus nidulans repressor CREA involved in carbon catabolite repression, forms several complexes with DNA fragments carrying the consensus 5'-SYGGRG-3'. These results suggest that a CREA homolog may be involved in the regulation of pg1. |
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| Keywords |
Amino Acid Sequence; Ascomycota/enzymology; Ascomycota/genetics; Aspergillus nidulans/metabolism; Base Sequence; Blotting, Northern; Consensus Sequence; Enzyme Repression; Fungal Proteins/biosynthesis; Fungal Proteins/metabolism; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Glucose/pharmacology; Glutathione Transferase/biosynthesis; Glutathione Transferase/metabolism; Molecular Sequence Data; Polygalacturonase/biosynthesis; Polygalacturonase/genetics; Promoter Regions, Genetic; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/metabolism; Repressor Proteins/biosynthesis; Repressor Proteins/metabolism; Restriction Mapping |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0044212: transcription regulatory region DNA binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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