PMID:8483416

Citation	Kulmburg, P, Mathieu, M, Dowzer, C, Kelly, J and Felenbok, B (1993) Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol. Microbiol. 7:847-57
Abstract	The CREA repressor responsible for carbon catabolite repression in Aspergillus nidulans represses the transcription of the ethanol regulon. The N-terminal part of the CREA protein encompassing the two zinc fingers (C2H2 class family) and an alanine-rich region was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase. Our results show that CREA is a DNA-binding protein able to bind to the promoters of both the specific trans-acting gene, alcR, and of the structural gene, alcA, encoding the alcohol dehydrogenase I. DNase I protection footprinting experiments revealed several specific binding sites in the alcR and in the alcA promoters having the consensus sequence 5'-G/CPyGGGG-3'. The disruption of one of these CREA-binding sites in the alcR promoter overlapping the induction target for the trans-activator ALCR results in a partially derepressed alc phenotype and derepressed alcR transcription, showing that this binding site is functional in vivo. Our data suggest that CREA represses the ethanol regulon by a double lock mechanism repressing both the trans-acting gene, alcR, and the structural gene, alcA.
Links	PubMed
Keywords	Alcohol Dehydrogenase/genetics; Aspergillus nidulans/drug effects; Aspergillus nidulans/genetics; Aspergillus nidulans/metabolism; Base Sequence; Binding Sites; Consensus Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Ethanol/pharmacology; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal/drug effects; Genes, Fungal; Glucose/pharmacology; Molecular Sequence Data; Promoter Regions, Genetic; Protein Binding; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Zinc Fingers/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
EMENI:CREA	enables	GO:0044212: transcription regulatory region DNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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