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PMID:8143847
| Citation |
Espeso, EA and Peñalva, MA (1994) In vitro binding of the two-finger repressor CreA to several consensus and non-consensus sites at the ipnA upstream region is context dependent. FEBS Lett. 342:43-8 |
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| Abstract |
The two zinc-fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'-SYGGRG-3'. We have determined all the CreA binding sites in a approximately 2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context-dependent manner; (ii) five non-consensus 6-bp sequences are also recognized by CreA; this non-canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged. |
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| Keywords |
Aspergillus nidulans/genetics; Aspergillus nidulans/metabolism; Base Composition; Base Sequence; Binding Sites; Consensus Sequence; DNA, Fungal/chemistry; DNA, Fungal/metabolism; Fungal Proteins/metabolism; Genes, Fungal; Molecular Sequence Data; Oligodeoxyribonucleotides/metabolism; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; Zinc Fingers |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0044212: transcription regulatory region DNA binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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