PMID:8126111

Citation	Abdel-Malek, Z, Swope, V, Collins, C, Boissy, R, Zhao, H and Nordlund, J (1993) Contribution of melanogenic proteins to the heterogeneous pigmentation of human melanocytes. J. Cell. Sci. 106 ( Pt 4):1323-31
Abstract	Human melanocytes from individuals with different skin types, as well as from the skin of the same individual, are heterogeneous in their melanin content. This heterogeneity may be attributed to differences in the activity and expression of the three melanogenic proteins: tyrosinase and tyrosinase-related proteins 1 and 2 (gp75 and DOPAchrome tautomerase, respectively), which in turn are affected by certain regulatory factors. Established melanocyte strains that exhibited intrinsic melanogenic heterogeneity could be separated into subpopulations according to density and melanin content by Percoll density gradient centrifugation. The least melanotic subpopulation consisted of melanocytes that contained an active tyrosinase enzyme and a low amount of melanin. Tyrosinase activity and the quantities of tyrosinase enzyme, tyrosinase-related protein-1 and DOPAchrome tautomerase gradually increased with increased melanin content and Percoll density of the isolated melanocyte subpopulations. We have found a direct correlation between melanin content, tyrosinase activity and the expression of the three melanogenic proteins in melanocyte strains established from different skin types. Addition of the two epidermal cytokines, tumor necrosis factor-alpha or interleukin-1 alpha, to cultures of human melanocytes from different skin types caused decreased proliferation, tyrosinase activity and expression of tyrosinase, tyrosinase-related protein-1 and DOPAchrome tautomerase. Similar results were obtained when Percoll-derived melanocyte subpopulations were treated with tumor necrosis factor-alpha and interleukin-1 alpha. These results indicate that the variation in melanin content in human melanocytes is due to differences in the activity and expression of the melanogenic proteins, which are influenced by autocrine and paracrine factors.
Links	PubMed
Keywords	Cell Separation/methods; Centrifugation, Density Gradient; Genetic Variation; Humans; Interleukin-1/pharmacology; Intramolecular Oxidoreductases; Isomerases/metabolism; Melanins/analysis; Melanocytes/drug effects; Melanocytes/physiology; Melanocytes/ultrastructure; Membrane Glycoproteins; Monophenol Monooxygenase/metabolism; Oxidoreductases; Proteins/metabolism; Skin Pigmentation/physiology; Specific Gravity; Tumor Necrosis Factor-alpha/pharmacology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:TYRO	involved_in	GO:0042438: melanin biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:TYRO		GO:0042438: melanin biosynthetic process	ECO:0000314:		P		Based on the results in fig 5.	complete CACAO 2234
HUMAN:TRPC1	involved_in	GO:0042438: melanin biosynthetic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
HUMAN:TRPC1		GO:0042438: melanin biosynthetic process	ECO:0000314:		P		Based on the results in fig 5.	complete CACAO 2235

References

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