PMID:2947981

Citation	Vergé, D, Daval, G, Marcinkiewicz, M, Patey, A, el Mestikawy, S, Gozlan, H and Hamon, M (1986) Quantitative autoradiography of multiple 5-HT1 receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats. J. Neurosci. 6:3474-82
Abstract	The distribution of the 2 main types (A and B) of 5-HT1 binding sites in the rat brain was studied by light-microscopic quantitative autoradiography. The 5-HT1A sites were identified using 3H-8-hydroxy-2-(N-dipropylamino)tetralin (3H-8-OH-DPAT) or 3H-5-HT as the ligand. In the latter case, it was shown that 3H-5-HT binding to 5-HT1A sites corresponded to that displaceable by 0.1 microM 8-OH-DPAT or 1 microM spiperone. The "non-5-HT1A" sites labeled by 3H-5-HT in the presence of 0.1 microM 8-OH-DPAT corresponded mainly to 5-HT1B sites. 5-HT1A binding was notably high in limbic regions (dentate gyrus, CA1 and CA3 hippocampal regions, lateral septum, frontal cortex), whereas 5-HT1B binding was particularly concentrated in extrapyramidal areas (caudate nucleus, globus pallidus, substantia nigra). Except in the latter regions, where only one class of 5-HT1 sites was found, both 5-HT1A and 5-HT1B sites existed in all areas examined. The selective degeneration of serotoninergic neurons produced by an intracerebral injection of 5,7-dihydroxytryptamine was associated only with a significant loss of 5-HT1A binding to the dorsal raphe nucleus (-60%) and of 5-HT1B binding to the substantia nigra (-37%). These results are discussed in relation to the possible identity of 5-HT1A and/or 5-HT1B sites with the presynaptic 5-HT autoreceptors controlling nerve impulse flow and neurotransmitter release in serotoninergic neurons.
Links	PubMed
Keywords	5,7-Dihydroxytryptamine/pharmacology; 8-Hydroxy-2-(di-n-propylamino)tetralin; Animals; Autoradiography; Brain/drug effects; Brain Chemistry; Caudate Nucleus/analysis; Choroid Plexus/analysis; Dihydroxytryptamines/pharmacology; Globus Pallidus/analysis; Limbic System/analysis; Male; Rats; Rats, Inbred Strains; Receptors, Serotonin/analysis; Receptors, Serotonin/metabolism; Reserpine/pharmacology; Serotonin/metabolism; Substantia Nigra/analysis; Tetrahydronaphthalenes/metabolism; Tissue Distribution
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:5HT1A	enables	GO:0008144: drug binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:5HT1A	enables	GO:0051378: serotonin binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
RAT:5HT1B	enables	GO:0051378: serotonin binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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