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PMID:28337219
| Citation |
Krejčí, J, Legartová, S and Bártová, E (2017) Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli. Stem Cells Int 2017:1021240 |
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| Abstract |
Cajal bodies (CBs) are important compartments containing accumulated proteins that preferentially regulate RNA-related nuclear events, including splicing. Here, we studied the nuclear distribution pattern of CBs in neurogenesis. In adult brains, coilin was present at a high density, but CB formation was absent in the nuclei of the choroid plexus of the lateral ventricles. Cells of the adult hippocampus were characterized by a crescent-like morphology of coilin protein. We additionally observed a 70 kDa splice variant of coilin in adult mouse brains, which was different to embryonic brains and mouse pluripotent embryonic stem cells (mESCs), characterized by the 80 kDa standard variant of coilin. Here, we also showed that depletion of coilin is induced during neural differentiation and HDAC1 deficiency in mESCs caused coilin accumulation inside the fibrillarin-positive region of the nucleoli. A similar distribution pattern was observed in adult brain hippocampi, characterized by lower levels of both coilin and HDAC1. In summary, we observed that neural differentiation and HDAC1 deficiency lead to coilin depletion and coilin accumulation in body-like structures inside the nucleoli. |
| Links |
PubMed PMC5350323 Online version:10.1155/2017/1021240 |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005634: nucleus |
ECO:0000314: |
C |
Figure 3. "Surprisingly, in olfactory bulbs (Figures 3(a)(A), 3(a)(B), and 3(b)(A)), high levels of coilin were noted in the highly DAPI-dense nuclear regions surrounding single CBs (Figures 3(b)(B)–3(b)(D)). This nuclear distribution pattern of coilin was observed in individual nuclei of the granular layer of the OBs in adult brain (Figures 3(b)(A)–3(b)(C); see magnification in Figure 3(b)(D) and quantification in Figure 3(b)(E))." Mouse, coilin. |
complete | ||||
Notes
See also
References
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