PMID:26459165

Citation	Callahan, CT, Wilson, KM and Ely, B (2016) Characterization of the Proteins Associated with Caulobacter crescentus Bacteriophage CbK Particles. Curr. Microbiol. 72:75-80
Abstract	Bacteriophage genomes contain an abundance of genes that code for hypothetical proteins with either a conserved domain or no predicted function. The Caulobacter phage CbK has an unusual shape, designated morphotype B3 that consists of an elongated cylindrical head and a long flexible tail. To identify CbK proteins associated with the phage particle, intact phage particles were subjected to SDS-PAGE, and the resulting protein bands were digested with trypsin and analyzed using MALDI mass spectroscopy to provide peptide molecular weights. These peptide molecular weights were then compared with the peptides that would be generated from the predicted amino acid sequences that are coded by the CbK genome, and the comparison of the actual and predicted peptide masses resulted in the identification of single genes that could code for the set of peptides derived from each of the 20 phage proteins. We also found that CsCl density gradient centrifugation resulted in the separation of empty phage heads, phage heads containing material organized in a spiral, isolated phage tails, and other particulate material from the intact phage particles. This additional material proved to be a good source of additional phage proteins, and preliminary results suggest that it may include a CbK DNA replication complex.
Links	PubMed PMC4703473 Online version:10.1007/s00284-015-0922-7
Keywords	Bacteriophages/chemistry; Bacteriophages/isolation & purification; Caulobacter crescentus/virology; Centrifugation, Density Gradient; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Viral Proteins/analysis; Viral Proteins/chemistry; Viral Proteins/isolation & purification
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:J3SMK2		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp270/putative-lectin like domain protein (an uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:J3UIK3		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp101/tail fiber protein (putative tail protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete CACAO 13567
9CAUD:J3SVM5		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp318/terminase large subunit (Intein-containing putative terminase large subunit) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete CACAO 13568
9CAUD:J3SKZ5		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp123/T7-like Pol I DNA Polymerase (putative T7-like Pol I DNA Polymerase) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1.	complete CACAO 13569
9CAUD:J3SKV6		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp273/Conserved phage protein (uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. These proteins were isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete CACAO 13570
9CAUD:K4JNV0		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp42/portal protein was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.	complete
9CAUD:K4K3Z1		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp137/Putative rIIb-like protein (uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:J3UIJ5		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp111/ Ribonucleoside diphosphate reductase beta subunit (Putative ribonucleoside diphosphate reductase beta subunit) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:J3SVS8		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp161/Nicotinate phosphoribosyltransferase (Putative lipoprotein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.	complete
9CAUD:J3U9K3		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp164/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1.	complete
9CAUD:J3SVU2		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp119/Transcription terminator factor Rho (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:J3SMP4		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp60/DHH phosphoesterase protein (Putative DHH phosphoesterase protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:K4JRJ4		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp68/Major capsid protein was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete
9CAUD:J3SKX8		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp180/Core domain of the SPFH superfamily (Putative band 7 lipoprotein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete
9CAUD:J3SKX4		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp198/HNH nuclease domain (Putative HNH endonuclease) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 1 and 2.	complete
9CAUD:J3U9T1		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp057/HD-domain/PDEase-like protein (Putative HD-domain/PDEase-like protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete CACAO 13742
9CAUD:J3SL15		GO:0019012: virion	ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion		C		In Table 1 gp048/Hypothetical protein (Uncharacterized protein) was shown to be associated with the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2 - denoted "10".	complete CACAO 13743
9CAUD:J3U9F3		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp242/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete
9CAUD:J3SML7		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp195/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete
9CAUD:J3SKV0		GO:0019012: virion	ECO:0005527:		C		In Table 1 gp303/Hypothetical protein (Uncharacterized protein) was shown to be part of the Caulobacter phage Cbk virus (also referenced as Caulobacter virus phiCbk) through MALDI-TOF MS analysis. This protein was isolated from the phage as shown through SDS-PAGE in Figure 2.	complete

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