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PMID:24356419
| Citation |
Zhu, J, Sheng, J, Dong, H, Kang, L, Ang, J and Xu, Z (2013) Phospholipid Scramblase 1 Functionally Interacts with Angiogenin and Regulates Angiogenin-Enhanced rRNA Transcription. Cell. Physiol. Biochem. 32:1695-706 |
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| Abstract |
Background: Angiogenin (ANG) can translocate to the target cell nucleus and accumulate in the nucleolus to enhance rRNA transcription, thus promoting cell proliferation. However, the regulation of ANG-enhanced rRNA transcription remains unknown. Previously we identified phospholipid scramblase 1 (PLSCR1) as a potential ANG-interacting protein in yeast two-hybrid screening. Methods: The interaction was re-confirmed in yeast cells and further verified by in vitro pull down, in vivo co-immunoprecipitation (Co-IP), fluorescent resonance energy transfer (FRET) and immunofluorescence analyses. The rRNA transcription level was determined by real-time quantitative PCR and Northern blot. Results: PLSCR1 was identified as a novel ANG-interacting protein. Notably, PLSCR1 interacted with ANG in the cell nucleus and regulated rRNA transcription. Furthermore, depletion of cellular ANG expression abolished PLSCR1-enhanced rRNA transcription, which could be rescued by exogenous ANG. Conclusion: Our data suggest that PLSCR1 positively regulates rRNA transcription through interacting with ANG, thus deepening our understanding on rRNA transcription regulation. © 2014 S. Karger AG, Basel. |
| Links |
PubMed Online version:10.1159/000356604 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005730: nucleolus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:1901838: positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter |
ECO:0000314: |
P |
Figure 3A-F provides data proving that HeLa cells that were transiently transfected with over-expression of PLSCR1 have higher levels of 47S pre-rRNA than HeLa cells transiently transfected with a control. |
complete | ||||
| GO:0005730: nucleolus |
ECO:0000314: |
C |
Figure 2A-C provides data confirming that ANG is in the nucleus of Hela cells. |
complete | ||||
| GO:0005730: nucleolus |
ECO:0000314: |
C |
Figure 2A provides data confirming that ANG is in the nucleus of Hela cells. |
complete | ||||
See also
References
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