PMID:24139936

Citation	Franczak, A, Wojciechowicz, B, Kolakowska, J and Kotwica, G' (2013) The effect of interleukin-1β, interleukin-6, and tumor necrosis factor-α on estradiol-17β release in the myometrium: The in vitro study on the pig model. Theriogenology '
Abstract	Estradiol-17β (E2) is a potent regulator of early pregnancy and the estrous cycle in pigs. Production of E2 occurs in the porcine myometrium, but the factors involved in its regulation are unknown. In this in vitro study, it was investigated whether interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α affect the release of E2 from the porcine myometrium on Days 10 to 11, 12 to 13, and 15 to 16 of pregnancy and the estrous cycle. The expression of the cytochrome P450 family 19 (CYP19) gene and the presence of the aromatase cytochrome P450 protein in the myometrium confirmed the ability of the tissue to produce E2. In gravid pigs, the expression of IL1RI mRNA and IL6R mRNA was markedly increased on Days 15 to 16 of gestation, whereas TNFRI mRNA was increased on Days 10 to 11 of gestation. In cyclic pigs, the expression of myometrial IL1RI mRNA did not differ among the studied days, although the expression of IL6R and TNFRI mRNAs was increased on Days 15 to 16. In gravid pigs, IL-1β, IL-6, and TNF-α increased myometrial E2 secretion on Days 15 to 16 but did not affect E2 release on Days 10 to 11 and 12 to 13 of pregnancy. In cyclic pigs, IL-1β, IL-6, and TNF-α did not increase myometrial E2 release. In conclusion, IL-1β, IL-6, and TNF-α affected myometrial E2 release in a manner that is dependent on the physiologic status of the female. The porcine myometrium expresses IL1RI, IL6R, and TNFRI genes and is the target tissue for IL-1β, IL-6, and TNF-α. In gravid pigs, IL-1β, IL-6, and TNF-α may increase myometrial release of E2in vitro specifically on Days 15 to 16 of pregnancy. These findings may be of interest to researchers using pigs as an animal model for fetal programming.
Links	PubMed Online version:10.1016/j.theriogenology.2013.09.024
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PIG:IL6	involved_in	GO:2000866: positive regulation of estradiol secretion	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
PIG:IL6		GO:2000866: positive regulation of estradiol secretion	ECO:0000314:		P		Day 15 and 16 of Pregnancy figure 4 show that increased IL-6 leads to increased estradiol 17beta secretion.	complete CACAO 8995
PIG:IL1B		GO:2000866: positive regulation of estradiol secretion	ECO:0000314:		P		Day 15 and 16 of Figure 4 show that increased IL-1beta resulted in an increase in estradiol 17 beta	complete CACAO 8996
PIG:IL1B	involved_in	GO:2000866: positive regulation of estradiol secretion	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
PIG:TNFA	involved_in	GO:2000866: positive regulation of estradiol secretion	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
PIG:TNFA		GO:2000866: positive regulation of estradiol secretion	ECO:0000314:		P		Figure 4.	complete CACAO 8997

References

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