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PMID:23494967

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Citation

Hu, J, Sun, S, Jiang, Q, Sun, S, Wang, W, Gui, Y and Song, H (2013) Yes-associated protein (yap) is required for early embryonic development in zebrafish (danio rerio). Int. J. Biol. Sci. 9:267-78

Abstract

The hippo (Hpo) signaling pathway plays a critical role in regulation of organ size. The kinase cascade ultimately antagonizes the transcriptional co-activator Yki/YAP, which is a key regulator of cell proliferation and apoptosis. In this study, we performed a knocking down study using antisense morpholino (MO) reagents and found that zebrafish YAP, a key transcriptional co-activator of Hpo pathway, plays a critical role in early embryonic development. At the cellular level, yap inhibition increases apoptosis and decreases cell proliferation. Reduction of yap function severely delays several developmental events, including gastrulation, cardiogenesis and hematopoiesis. Knockdown of yap showed some evidence of ventralization, including reduction of dorsally expressed marker goosecoid (gsc), expansion of ventral marker gata2, disruption of the somites, and reduction in head size. Finally, we performed a preliminary analysis with real-time polymerase chain reaction (qPCR) for the candidate targets of zebrafish Hpo pathway. In conclusion, our results revealed that zebrafish yap coordinately regulates cell proliferation and apoptosis and is required for dorsoventral axis formation, gastrulation, cardiogenesis, hematopoiesis, and somitogenesis.

Links

PubMed PMC3596712 Online version:10.7150/ijbs.4887

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:Q1L8J7

involved_in

GO:0060914: heart formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q1L8J7

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q1L8J7

involved_in

GO:0009950: dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q1L8J7

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:Q1L8J7

involved_in

GO:0010629: negative regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:YAP1

GO:0035162: embryonic hemopoiesis

ECO:0000315:

P

Figure 8, yap knockdowns have delayed maturation of red blood cells.

complete
CACAO 7060

DANRE:YAP1

GO:0060914: heart formation

ECO:0000315:

P

Figure 7, yap knockdowns show delay in cardiogenesis.

complete
CACAO 7061

DANRE:YAP1

GO:0009950: dorsal/ventral axis specification

ECO:0000315:

P

Figure 5, yap knockdown leads to reduced dorsoventral marker expression.

complete
CACAO 7062

DANRE:YAP1

GO:0010629: negative regulation of gene expression

ECO:0000315:

P

Figure 6, yap knockdowns have increased expression of myoD.

complete
CACAO 7063

DANRE:YAP1

GO:0043066: negative regulation of apoptotic process

ECO:0000315:

P

Figure 4, yap knockdowns show increased cell death

complete
CACAO 7064

DANRE:YAP1

involved_in

GO:0060914: heart formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:YAP1

involved_in

GO:0035162: embryonic hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:YAP1

involved_in

GO:0009950: dorsal/ventral axis specification

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:YAP1

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:YAP1

involved_in

GO:0010629: negative regulation of gene expression

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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