PMID:23494967

Citation	Hu, J, Sun, S, Jiang, Q, Sun, S, Wang, W, Gui, Y and Song, H (2013) Yes-associated protein (yap) is required for early embryonic development in zebrafish (danio rerio). Int. J. Biol. Sci. 9:267-78
Abstract	The hippo (Hpo) signaling pathway plays a critical role in regulation of organ size. The kinase cascade ultimately antagonizes the transcriptional co-activator Yki/YAP, which is a key regulator of cell proliferation and apoptosis. In this study, we performed a knocking down study using antisense morpholino (MO) reagents and found that zebrafish YAP, a key transcriptional co-activator of Hpo pathway, plays a critical role in early embryonic development. At the cellular level, yap inhibition increases apoptosis and decreases cell proliferation. Reduction of yap function severely delays several developmental events, including gastrulation, cardiogenesis and hematopoiesis. Knockdown of yap showed some evidence of ventralization, including reduction of dorsally expressed marker goosecoid (gsc), expansion of ventral marker gata2, disruption of the somites, and reduction in head size. Finally, we performed a preliminary analysis with real-time polymerase chain reaction (qPCR) for the candidate targets of zebrafish Hpo pathway. In conclusion, our results revealed that zebrafish yap coordinately regulates cell proliferation and apoptosis and is required for dorsoventral axis formation, gastrulation, cardiogenesis, hematopoiesis, and somitogenesis.
Links	PubMed PMC3596712 Online version:10.7150/ijbs.4887
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DANRE:Q1L8J7	involved_in	GO:0060914: heart formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:Q1L8J7	involved_in	GO:0035162: embryonic hemopoiesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:Q1L8J7	involved_in	GO:0009950: dorsal/ventral axis specification	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:Q1L8J7	involved_in	GO:0043066: negative regulation of apoptotic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:Q1L8J7	involved_in	GO:0010629: negative regulation of gene expression	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:YAP1		GO:0035162: embryonic hemopoiesis	ECO:0000315:		P		Figure 8, yap knockdowns have delayed maturation of red blood cells.	complete CACAO 7060
DANRE:YAP1		GO:0060914: heart formation	ECO:0000315:		P		Figure 7, yap knockdowns show delay in cardiogenesis.	complete CACAO 7061
DANRE:YAP1		GO:0009950: dorsal/ventral axis specification	ECO:0000315:		P		Figure 5, yap knockdown leads to reduced dorsoventral marker expression.	complete CACAO 7062
DANRE:YAP1		GO:0010629: negative regulation of gene expression	ECO:0000315:		P		Figure 6, yap knockdowns have increased expression of myoD.	complete CACAO 7063
DANRE:YAP1		GO:0043066: negative regulation of apoptotic process	ECO:0000315:		P		Figure 4, yap knockdowns show increased cell death	complete CACAO 7064
DANRE:YAP1	involved_in	GO:0060914: heart formation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:YAP1	involved_in	GO:0035162: embryonic hemopoiesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:YAP1	involved_in	GO:0009950: dorsal/ventral axis specification	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:YAP1	involved_in	GO:0043066: negative regulation of apoptotic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DANRE:YAP1	involved_in	GO:0010629: negative regulation of gene expression	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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