PMID:22492707

Citation	Tsai, SP, Su, GC, Lin, SW, Chung, CI, Xue, X, Dunlop, MH, Akamatsu, Y, Jasin, M, Sung, P and Chi, P (2012) Rad51 presynaptic filament stabilization function of the mouse Swi5-Sfr1 heterodimeric complex. Nucleic Acids Res. 40:6558-69
Abstract	Homologous recombination (HR) represents a major error-free pathway to eliminate pre-carcinogenic chromosomal lesions. The DNA strand invasion reaction in HR is mediated by a helical filament of the Rad51 recombinase assembled on single-stranded DNA that is derived from the nucleolytic processing of the primary lesion. Recent studies have found that the human and mouse Swi5 and Sfr1 proteins form a complex that influences Rad51-mediated HR in cells. Here, we provide biophysical evidence that the mouse Swi5-Sfr1 complex has a 1:1 stoichiometry. Importantly, the Swi5-Sfr1 complex, but neither Swi5 nor Sfr1 alone, physically interacts with Rad51 and stimulates Rad51-mediated homologous DNA pairing. This stimulatory effect stems from the stabilization of the Rad51-ssDNA presynaptic filament. Moreover, we provide evidence that the RSfp (rodent Sfr1 proline rich) motif in Sfr1 serves as a negative regulatory element. These results thus reveal an evolutionarily conserved function in the Swi5-Sfr1 complex and furnish valuable information as to the regulatory role of the RSfp motif that isspecific to themammalianSfr1 orthologs.
Links	PubMed PMC3413116 Online version:10.1093/nar/gks305
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:SWI5		GO:0000730: DNA recombinase assembly	ECO:0000270:		P		Fig. 2 shows that the SWI5-SFR1 complex functions in stabilization of the Rad51 filament	complete CACAO 4867
MOUSE:SFR1		GO:0000730: DNA recombinase assembly	ECO:0000270:		P		Fig. 2 shows the SWI5-SFR1 complex functions in stabilizing the Rad51 filament	complete CACAO 4868

References

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