PMID:22138402

Citation	Wang, HQ, Xu, ML, Ma, J, Zhang, Y and Xie, CH (2012) Frizzled-8 as a putative therapeutic target in human lung cancer. Biochem. Biophys. Res. Commun. 417:62-6
Abstract	Lung cancer is the leading cause of cancer related deaths worldwide. It is necessary to better understand the molecular mechanisms involved in lung cancer in order to develop more effective therapeutics for the treatment of this disease. Recent reports have shown that Wnt signaling pathway is important in a number of cancer types including lung cancer. However, the role of Frizzled-8 (Fzd-8), one of the Frizzled family of receptors for the Wnt ligands, in lung cancer still remains to be elucidated. Here in this study we showed that Fzd-8 was over-expressed in human lung cancer tissue samples and cell lines. To investigate the functional importance of the Fzd-8 over-expression in lung cancer, we used shRNA to knock down Fzd-8 mRNA in lung cancer cells expressing the gene. We observed that Fzd-8 shRNA inhibited cell proliferation along with decreased activity of Wnt pathway in vitro, and also significantly suppressed A549 xenograft model in vivo (p<0.05). Furthermore, we found that knocking down Fzd-8 by shRNA sensitized the lung cancer cells to chemotherapy Taxotere. These data suggest that Fzd-8 is a putative therapeutic target for human lung cancer and over-expression of Fzd-8 may be important for aberrant Wnt activation in lung cancer.
Links	PubMed Online version:10.1016/j.bbrc.2011.11.055
Keywords	Animals; Antineoplastic Agents/therapeutic use; Cell Line, Tumor; Cell Proliferation; Drug Resistance, Neoplasm/genetics; Gene Knockdown Techniques; Humans; Lung Neoplasms/drug therapy; Mice; RNA, Small Interfering/genetics; Receptors, Cell Surface/antagonists & inhibitors; Receptors, Cell Surface/genetics; Taxoids/therapeutic use; Xenograft Model Antitumor Assays
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:FZD8		GO:0060070: canonical Wnt receptor signaling pathway	ECO:0000315:		P		Figure 2	complete CACAO 7816
HUMAN:FZD8	involved_in	GO:0060070: canonical Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.