PMID:22024163

Citation	Petsalaki, E, Akoumianaki, T, Black, EJ, Gillespie, DA and Zachos, G (2011) Phosphorylation at serine 331 is required for Aurora B activation. J. Cell Biol. 195:449-66
Abstract	Aurora B kinase activity is required for successful cell division. In this paper, we show that Aurora B is phosphorylated at serine 331 (Ser331) during mitosis and that phosphorylated Aurora B localizes to kinetochores in prometaphase cells. Chk1 kinase is essential for Ser331 phosphorylation during unperturbed prometaphase or during spindle disruption by taxol but not nocodazole. Phosphorylation at Ser331 is required for optimal phosphorylation of INCENP at TSS residues, for Survivin association with the chromosomal passenger complex, and for complete Aurora B activation, but it is dispensable for Aurora B localization to centromeres, for autophosphorylation at threonine 232, and for association with INCENP. Overexpression of Aurora B(S331A), in which Ser331 is mutated to alanine, results in spontaneous chromosome missegregation, cell multinucleation, unstable binding of BubR1 to kinetochores, and impaired mitotic delay in the presence of taxol. We propose that Chk1 phosphorylates Aurora B at Ser331 to fully induce Aurora B kinase activity. These results indicate that phosphorylation at Ser331 is an essential mechanism for Aurora B activation.
Links	PubMed PMC3206340 Online version:10.1083/jcb.201104023
Keywords	Amino Acid Sequence; Animals; CHO Cells; Chromosomal Proteins, Non-Histone/metabolism; Chromosome Segregation; Cricetinae; Enzyme Activation; Humans; Kinetochores/metabolism; Microscopy, Fluorescence; Mitosis; Molecular Sequence Data; Paclitaxel/pharmacology; Phosphorylation; Protein Binding; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Serine/genetics; Serine/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:AURKB	part_of	GO:0000776: kinetochore	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
HUMAN:AURKB		GO:0000776: kinetochore	ECO:0000314:		C		Figure 2	complete CACAO 6579
HUMAN:CHK1		GO:0004672: protein kinase activity	ECO:0000315:		F		Figure 1	complete CACAO 6583
HUMAN:CHK1	enables	GO:0004672: protein kinase activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete

References

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