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PMID:22024163

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Citation

Petsalaki, E, Akoumianaki, T, Black, EJ, Gillespie, DA and Zachos, G (2011) Phosphorylation at serine 331 is required for Aurora B activation. J. Cell Biol. 195:449-66

Abstract

Aurora B kinase activity is required for successful cell division. In this paper, we show that Aurora B is phosphorylated at serine 331 (Ser331) during mitosis and that phosphorylated Aurora B localizes to kinetochores in prometaphase cells. Chk1 kinase is essential for Ser331 phosphorylation during unperturbed prometaphase or during spindle disruption by taxol but not nocodazole. Phosphorylation at Ser331 is required for optimal phosphorylation of INCENP at TSS residues, for Survivin association with the chromosomal passenger complex, and for complete Aurora B activation, but it is dispensable for Aurora B localization to centromeres, for autophosphorylation at threonine 232, and for association with INCENP. Overexpression of Aurora B(S331A), in which Ser331 is mutated to alanine, results in spontaneous chromosome missegregation, cell multinucleation, unstable binding of BubR1 to kinetochores, and impaired mitotic delay in the presence of taxol. We propose that Chk1 phosphorylates Aurora B at Ser331 to fully induce Aurora B kinase activity. These results indicate that phosphorylation at Ser331 is an essential mechanism for Aurora B activation.

Links

PubMed PMC3206340 Online version:10.1083/jcb.201104023

Keywords

Amino Acid Sequence; Animals; CHO Cells; Chromosomal Proteins, Non-Histone/metabolism; Chromosome Segregation; Cricetinae; Enzyme Activation; Humans; Kinetochores/metabolism; Microscopy, Fluorescence; Mitosis; Molecular Sequence Data; Paclitaxel/pharmacology; Phosphorylation; Protein Binding; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Serine/genetics; Serine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:AURKB

part_of

GO:0000776: kinetochore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:AURKB

GO:0000776: kinetochore

ECO:0000314:

C

Figure 2

complete
CACAO 6579

HUMAN:CHK1

GO:0004672: protein kinase activity

ECO:0000315:

F

Figure 1

complete
CACAO 6583

HUMAN:CHK1

enables

GO:0004672: protein kinase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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