PMID:21637952

Citation	Xiong, F, Lin, Y, Han, Z, Shi, G, Tian, L, Wu, X, Zeng, Q, Zhou, Y, Deng, J and Chen, H (2012) Plk1-mediated phosphorylation of UAP56 regulates the stability of UAP56. Mol. Biol. Rep. 39:1935-42
Abstract	Polo-like kinase 1 (Plk1) is a conserved serine/threonine protein kinase that plays pivotal roles during the cell cycle and cell proliferation. Although a number of important targets have been identified, the mechanism of Plk1-regulated pathways and the bulk of the Plk1 interactome are largely unknown. Here, we demonstrate that Plk1 interacts with the DExH/D RNA helicase, UAP56. The protein levels of UAP56 and Plk1 are inversely correlated during the cell cycle. We also show that Plk1 phosphorylates UAP56 in vitro and in vivo and that Plk1-dependent phosphorylation of UAP56 triggers ubiquitination and degradation of UAP56 through proteasomes. This result suggests that Plk1-mediated phosphorylation of UAP56 regulates the stability of UAP56. Our results will be helpful in further understanding mRNA metabolism, cell cycle progression, and the link between mRNA metabolism and cellular function.
Links	PubMed Online version:10.1007/s11033-011-0940-x
Keywords	Animals; Cell Cycle Proteins/metabolism; Cell Line, Tumor; DEAD-box RNA Helicases/metabolism; Flow Cytometry; HEK293 Cells; Humans; Immunoprecipitation; Mice; Phosphorylation; Protein Stability; Protein-Serine-Threonine Kinases/metabolism; Proto-Oncogene Proteins/metabolism; RNA, Small Interfering/genetics; Transfection; Two-Hybrid System Techniques
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PANTR:DX39B		GO:0051095: regulation of helicase activity	ECO:0000315:		P		Fig 3(a) - UAP56 appear to become unstable when Plk1 was most abundant. Fig 3(b) - depletion of Plk1 dramatically increased the stability of UAP56.	complete CACAO 11747
MOUSE:PLK1		GO:0004672: protein kinase activity	ECO:0000314:		F		Fig 2(a) - Wild type Plk1 phosphorylates UAP56.	complete CACAO 11751
MOUSE:PLK1		GO:0006468: protein phosphorylation	ECO:0000315:		P		Fig 2(b) - Phosphorylation of UAP56 is dependent upon levels of Plk1.	complete CACAO 11752

References

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