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PMID:21567317
| Citation |
Massey, S, Quiñones, B and Teter, K (2011) A cell-based fluorescent assay to detect the activity of Shiga toxin and other toxins that inhibit protein synthesis. Methods Mol. Biol. 739:49-59 |
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| Abstract |
Escherichia coli O157:H7, a major cause of food-borne illness, produces Shiga toxins (Stxs) that block protein synthesis by inactivating the ribosome. In this chapter, we describe a simple cell-based fluorescent assay to detect Stxs and inhibitors of toxin activity. The assay can also be used to detect other plant and bacterial toxins that arrest protein synthesis. |
| Links |
PubMed Online version:10.1007/978-1-61779-102-4_5 |
| Keywords |
Animals; Cercopithecus aethiops; Escherichia coli O157/isolation & purification; Escherichia coli O157/pathogenicity; Food Contamination/analysis; Food Safety; Green Fluorescent Proteins/biosynthesis; Protein Biosynthesis; Shiga Toxin 2/analysis; Shiga Toxin 2/antagonists & inhibitors; Vero Cells |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031640 : killing of cells of other organism |
ECO:0000314: |
P |
Figure 3. |
complete | ||||
| GO:0031640 : killing of cells of other organism |
ECO:0000314: |
P |
Figure 3. |
complete | ||||
| GO:0031640 : killing of cells of other organism |
ECO:0000314: |
P |
Figure 1. |
complete | ||||
See also
References
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