PMID:2104611

Citation	Errington, J and Vogt, CH (1990) Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator. J. Bacteriol. 172:488-90
Abstract	We have isolated mutations that appear to inactivate the gene (lacA) encoding an endogenous beta-galactosidase activity in Bacillus subtilis and in a closely linked negative regulatory element (lacR). Both genes map to the hisA-thrA region. The lacA mutations may help to avoid some of the problems arising from the use of the Escherichia coli lacZ gene as a reporter gene in B. subtilis.
Links	PubMed PMC208459
Keywords	Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Chromosome Mapping; Galactosidases/genetics; Genes, Bacterial; Genes, Regulator; Mutation; beta-Galactosidase/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:BGAL2		GO:0004565: beta-galactosidase activity	ECO:0000316:	PMID:2104611	F		Figure 1 shows that lacR is responsible for regulating beta-galactosidase activity and the lacR1 mutation upregulates beta-galactosidase activity. Figure 2 shows that when the same mutation is applied along with the lacA17 mutation, beta-galactosidase activity decreases showing that lacA is directly responsible for beta-galactosidase activity and is regulated by lacR.	complete
BACSU:GANR		GO:0006355: regulation of transcription, DNA-dependent	ECO:0000315:		P		Figure 1 shows the comparison of beta-galactosidase activity for mutant and wild type lacR strains. This shows that lacR is a repressor gene for genes responsible for beta-galactosidase activity.	complete

References

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