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PMID:19220469
| Citation |
Saenkham, P, Vattanaviboon, P and Mongkolsuk, S (2009) Mutation in sco affects cytochrome c assembly and alters oxidative stress resistance in Agrobacterium tumefaciens. FEMS Microbiol. Lett. 293:122-9 |
|---|---|
| Abstract |
Sco (for the synthesis of cytochrome c oxidase) is a mitochondrial membrane protein essential for the correct assembly of cytochrome c oxidase. sco homolog genes exist in a wide variety of bacterial species. Inactivation of Agrobacterium tumefaciens sco leads to markedly decreased cytochrome c oxidase activity. This phenotype can be complemented by either supplementing the culture medium with copper or by a plasmid containing sco. The sco mutant also alters resistance to a superoxide generator menadione and H2O2. Mutational analysis of conserved cysteine residues and a histidine residue within the putative copper ions binding motif of Sco indicates that these residues are essential for the biological activity of Sco. To summarize, we find that A. tumefaciens sco is required for the delivery of copper into active cytochrome c oxidase and in maintaining optimal resistance levels to oxidative stress. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2009.01516.x |
| Keywords |
Agrobacterium tumefaciens/enzymology; Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/metabolism; Agrobacterium tumefaciens/physiology; Amino Acid Motifs; Copper/metabolism; Electron Transport Complex IV/metabolism; Heat-Shock Response; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitochondria/metabolism; Mitochondrial Proteins/chemistry; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Mutagenesis, Site-Directed; Mutation; Oxidative Stress |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006461: protein complex assembly |
ECO:0000315: |
P |
Figure 2 supports the function of the Sco1 gene by showing that it is essential for Cytochrome c oxidase activity. |
complete | ||||
| GO:0006878: cellular copper ion homeostasis |
ECO:0000315: |
P |
Figure 2 supports the function of the Sco1 gene by showing that it is essential for Cytochrome c oxidase activity. |
complete | ||||
| GO:0004129: cytochrome-c oxidase activity |
ECO:0000315: |
F |
Figure 2 supports the function of the Sco1 gene by showing that it is essential for Cytochrome c oxidase activity. |
complete | ||||
See also
References
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