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PMID:16542652
| Citation |
Arden, C, Baltrusch, S and Agius, L (2006) Glucokinase regulatory protein is associated with mitochondria in hepatocytes. FEBS Lett. 580:2065-70 |
|---|---|
| Abstract |
The association of glucokinase with liver mitochondria has been reported [Danial et al. (2003) BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424, 952-956]. We confirmed association of glucokinase immunoreactivity with rat liver mitochondria using Percoll gradient centrifugation and demonstrated its association with the 68 kDa regulatory protein (GKRP) but not with the binding protein phosphofructokinase-2/fructose bisphosphatase-2. Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP. Combined with previous observations that GKRP overexpression partially inhibits glycolysis [de la Iglesia et al. (2000) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the hepatocyte. J. Biol. Chem. 275, 10597-10603] these findings suggest that there may be distinct glycolytic pools of glucokinase. |
| Links |
PubMed Online version:10.1016/j.febslet.2006.03.009 |
| Keywords |
Animals; Carrier Proteins/metabolism; Cell Nucleus/metabolism; Cells, Cultured; Fructose-Bisphosphatase/metabolism; Fructosephosphates/metabolism; Glucagon/pharmacology; Glucokinase/metabolism; Hepatocytes/cytology; Hepatocytes/drug effects; Hepatocytes/enzymology; Hepatocytes/metabolism; Hexokinase/metabolism; Male; Mitochondria, Liver/drug effects; Mitochondria, Liver/enzymology; Mitochondria, Liver/metabolism; Phosphofructokinase-2/metabolism; Protein Binding; Protein Transport/drug effects; Rats; Rats, Wistar; Substrate Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005829: cytosol |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739: mitochondrion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005739: mitochondrion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005829: cytosol |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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