PMID:16436752

Citation	Maisetta, G, Batoni, G, Esin, S, Florio, W, Bottai, D, Favilli, F and Campa, M (2006) In vitro bactericidal activity of human beta-defensin 3 against multidrug-resistant nosocomial strains. Antimicrob. Agents Chemother. 50:806-9
Abstract	The antimicrobial activity of human beta-defensin 3 (hBD-3) against multidrug-resistant clinical isolates of Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Acinetobacter baumannii was evaluated. A fast bactericidal effect (within 20 min) against all bacterial strains tested was observed. The presence of 20% human serum abolished the bactericidal activity of hBD-3 against gram-negative strains and reduced the activity of the peptide against gram-positive strains.
Links	PubMed PMC1366902 Online version:10.1128/AAC.50.2.806-809.2006
Keywords	Bacteria/drug effects; Cross Infection/microbiology; Drug Resistance, Multiple, Bacterial; Humans; Serum/physiology; beta-Defensins/pharmacology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:D103A		GO:0042742: defense response to bacterium	ECO:0000314:		P		The bactericidal activity of hBD-3, an abbreviation of human beta-defensin 3 which is an alternative name for human beta-defensin 103, against various clinical isolates was evaluated by a liquid microdilution assay as shown in Figure 1. The results showed that hBD-3 used at a concentration of 4 μg/ml was bactericidal against A. baumannii (strain 3) after 3 min of incubation. hBD-3 used at a concentration of 8 μg/ml caused a bactericidal effect within 20 min of incubation against S. aureus (strain 3), E. faecium (strain 3), and P. aeruginosa (strain 3) and within 1 min against S. maltophilia (strain 4) and A. baumannii. At a concentration of 16 μg/ml, the peptide produced a bactericidal effect against S. aureus, E. faecium, P. aeruginosa, and S. maltophilia within 10, 20, 5, and 1 min, respectively.	complete CACAO 12386
HUMAN:D103A		GO:0051873: killing by host of symbiont cells	ECO:0000314:		P		Human Beta-defensin 103, also known as human beta-defensin 3 (hBD-3), has the ability to kill cells of other organisms. The bactericidal activity of hBD-3 against various clinical isolates was evaluated by a liquid microdilution assay as shown in Figure 1. The results of that assay showed that hBD-3 used at a concentration of 4 μg/ml was bactericidal against A. baumannii (strain 3) after 3 min of incubation. hBD-3 used at a concentration of 8 μg/ml caused a bactericidal effect within 20 min of incubation against S. aureus (strain 3), E. faecium (strain 3), and P. aeruginosa (strain 3) and within 1 min against S. maltophilia (strain 4) and A. baumannii. At a concentration of 16 μg/ml, the peptide produced a bactericidal effect against S. aureus, E. faecium, P. aeruginosa, and S. maltophilia within 10, 20, 5, and 1 min, respectively.	complete CACAO 12520
HUMAN:D103A		GO:0002376: immune system process	ECO:0000314:		P		Human Beta-defensin 103, also known as human beta-defensin 3 (hBD-3), plays a role in immune system processes. The bactericidal immune activity of hBD-3 against various clinical isolates was evaluated by a liquid microdilution assay as shown in Figure 1. The results of that assay showed that hBD-3 used at a concentration of 4 μg/ml was bactericidal against A. baumannii (strain 3) after 3 min of incubation. hBD-3 used at a concentration of 8 μg/ml caused a bactericidal effect within 20 min of incubation against S. aureus (strain 3), E. faecium (strain 3), and P. aeruginosa (strain 3) and within 1 min against S. maltophilia (strain 4) and A. baumannii. At a concentration of 16 μg/ml, the peptide produced a bactericidal effect against S. aureus, E. faecium, P. aeruginosa, and S. maltophilia within 10, 20, 5, and 1 min, respectively.	complete CACAO 12523
HUMAN:D103A		GO:0016045: detection of bacterium	ECO:0000314:		P		Human Beta-defensin 103, also known as human beta-defensin 3 (hBD-3), has the ability to detect bacterial cells and express bactericidal activity against them. The bactericidal activity of hBD-3 against various clinical isolates was evaluated by a liquid microdilution assay as shown in Figure 1. The results of that assay showed that hBD-3 used at a concentration of 4 μg/ml was bactericidal against A. baumannii (strain 3) after 3 min of incubation. hBD-3 used at a concentration of 8 μg/ml caused a bactericidal effect within 20 min of incubation against S. aureus (strain 3), E. faecium (strain 3), and P. aeruginosa (strain 3) and within 1 min against S. maltophilia (strain 4) and A. baumannii. At a concentration of 16 μg/ml, the peptide produced a bactericidal effect against S. aureus, E. faecium, P. aeruginosa, and S. maltophilia within 10, 20, 5, and 1 min, respectively.	complete CACAO 12524

References

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