PMID:15670776

Citation	Lai, YP, Peng, YF, Zuo, Y, Li, J, Huang, J, Wang, LF and Wu, ZR (2005) Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide. Biochem. Biophys. Res. Commun. 328:243-50
Abstract	Antimicrobial peptides from human skin are an important component of the innate immune response and play a key role as a first line of defense against infections. One such peptide is the recently discovered dermcidin-1L. To better understand its mechanism and to further investigate its antimicrobial spectrum, recombinant dermcidin-1L was expressed in Escherichia coli as a fusion protein and purified by affinity chromatography. The fusion protein was cleaved by factor Xa protease to produce recombinant dermcidin-1L. Antimicrobial and hemolytic assays demonstrated that dermcidin-1L displayed microbicidal activity against several opportunistic nosocomial pathogens, but no hemolytic activity against human erythrocytes even at concentrations up to 100 microM. Structural studies performed by circular dichroism spectroscopy indicated that the secondary structure of dermcidin-1L was very flexible, and both alpha-helix and beta-sheet structures might be required for the antimicrobial activity. Our results confirmed previous findings indicating that dermcidin-1L could have promising therapeutic potentials and shed new light on the structure-function relationship of dermcidin-1L.
Links	PubMed Online version:10.1016/j.bbrc.2004.12.143
Keywords	Antimicrobial Cationic Peptides/chemistry; Antimicrobial Cationic Peptides/genetics; Antimicrobial Cationic Peptides/pharmacology; Bacteria/cytology; Bacteria/drug effects; Dose-Response Relationship, Drug; Escherichia coli/genetics; Escherichia coli/metabolism; Hemolysis/drug effects; Humans; Lethal Dose 50; Molecular Weight; Peptides/chemistry; Peptides/genetics; Peptides/pharmacology; Protein Conformation; Protein Structure, Secondary; Recombinant Proteins/chemistry; Recombinant Proteins/pharmacology; Structure-Activity Relationship
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:DCD		GO:0042742: defense response to bacterium	ECO:0000314:		P		The antimicrobial activity of DCD-1, in the form of purified rDCD-1L, was assessed using a microbicidal assay in vitro against five different microorganisms. rDCD-1L was show to be active against E. coli CMCC(B)44102, P. sputita, S. aureus CMCC(B)26003, and S. aureus293 with 50% of bacteria killed at the concentrations of 1.8, 0.5, 8.2, and 17.8 μg/mL, respectively (Figure 5).	complete CACAO 12381
HUMAN:DCD		GO:0051873: killing by host of symbiont cells	ECO:0000314:		P		Human dermicidin, DCD-1, has the ability to kill cells of other organisms. The antimicrobial activity of DCD-1, in the form of purified rDCD-1L, was assessed using a microbicidal assay in vitro against five different microorganisms. rDCD-1L was show to be active against E. coli CMCC(B)44102, P. sputita, S. aureus CMCC(B)26003, and S. aureus293 with 50% of bacteria killed at the concentrations of 1.8, 0.5, 8.2, and 17.8 μg/mL, respectively (Figure 5).	complete CACAO 12512
HUMAN:DCD		GO:0002376: immune system process	ECO:0000314:		P		Human dermicidin, DCD-1, plays a role in immune system processes. The antimicrobial immune system activity of DCD-1, in the form of purified rDCD-1L, was assessed using a microbicidal assay in vitro against five different microorganisms. rDCD-1L was show to be active against E. coli CMCC(B)44102, P. sputita, S. aureus CMCC(B)26003, and S. aureus293 with 50% of bacteria killed at the concentrations of 1.8, 0.5, 8.2, and 17.8 μg/mL, respectively (Figure 5).	complete CACAO 12513
HUMAN:DCD		GO:0016045: detection of bacterium	ECO:0000314:		P		Human dermicidin, DCD-1, has the ability to detect bacterial cells and express antimicrobial activity against them. The antimicrobial activity of DCD-1, in the form of purified rDCD-1L, was assessed using a microbicidal assay in vitro against five different microorganisms. rDCD-1L was show to be active against E. coli CMCC(B)44102, P. sputita, S. aureus CMCC(B)26003, and S. aureus293 with 50% of bacteria killed at the concentrations of 1.8, 0.5, 8.2, and 17.8 μg/mL, respectively (Figure 5).	complete CACAO 12514

References

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