PMID:15647454

Citation	Patak, E, Pinto, FM, Story, ME, Pintado, CO, Fleming, A, Page, NM, Pennefather, JN and Candenas, ML (2005) Functional and molecular characterization of tachykinins and tachykinin receptors in the mouse uterus. Biol. Reprod. 72:1125-33
Abstract	The aim of this study was to analyze the function and expression of tachykinins, tachykinin receptors, and neprilysin (NEP) in the mouse uterus. A previous study showed that the uterotonic effects of substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) in estrogen-treated mice were mainly mediated by the tachykinin NK1 receptor. In the present work, further contractility studies were undertaken to determine the nature of the receptors mediating responses to tachykinins in uteri of late pregnant mice. Endpoint and real-time quantitative RT-PCR were used to analyze the expression of the genes that encode the tachykinins SP/NKA, NKB, and hemokinin-1 (HK-1) (Tac1, Tac2, and Tac4); and the genes that encode tachykinin NK1 (Tacr1), NK2 (Tacr2), and NK3 (Tacr3) receptors in uteri from pregnant and nonpregnant mice. The data show that the mRNAs of tachykinins (particularly NKB and HK-1), tachykinin receptors, and NEP are locally expressed in the mouse uterus, and their expression changes during the estrous cycle and during pregnancy. The tachykinin NK1 receptor is the predominant tachykinin receptor in the nonpregnant and early pregnant mouse and may mediate tachykinin-induced uterine contractions in the nonpregnant mouse. The tachykinin NK2 receptor is predominant in the late pregnant mouse and is the main receptor mediating uterotonic responses to tachykinins at late pregnancy. The tachykinin NK3 receptor is expressed in considerable amounts only in uteri from nonpregnant diestrous animals, and its physiological significance remains to be clarified.
Links	PubMed Online version:10.1095/biolreprod.104.036814
Keywords	Animals; Base Sequence; Estrus/genetics; Estrus/physiology; Female; Gene Expression; Mice; Mice, Inbred BALB C; Neprilysin/genetics; Neprilysin/physiology; Pregnancy; Pregnancy, Animal/genetics; Pregnancy, Animal/physiology; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, Tachykinin/agonists; Receptors, Tachykinin/antagonists & inhibitors; Receptors, Tachykinin/genetics; Receptors, Tachykinin/physiology; Tachykinins/genetics; Tachykinins/physiology; Uterine Contraction/genetics; Uterine Contraction/physiology; Uterus/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:NK1R		GO:0070472: positive regulation of uterine smooth muscle contraction	ECO:0000314:		P		from figure 1 increasing amounts of NK1 were added to the uterine preparation prior to testing. Contractile force produced by the longitudinally oriented smooth muscle fibers was recorded by a Grass FT03 force transducer connected to a MacLab data acquisition system. Uterine preparations treated with larger amounts of NK1 displayed increased levels of contraction.	complete CACAO 1965
MOUSE:NK1R	involved_in	GO:0070472: regulation of uterine smooth muscle contraction	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:NK2R	involved_in	GO:0070472: regulation of uterine smooth muscle contraction	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:NK2R		GO:0070472: positive regulation of uterine smooth muscle contraction	ECO:0000314:		P		from figure 1 increasing amounts of NK2 were added to the uterine preparation prior to testing. Contractile force produced by the longitudinally oriented smooth muscle fibers was recorded by a Grass FT03 force transducer connected to a MacLab data acquisition system. Uterine preparations treated with larger amounts of NK2 displayed increased levels of contraction.	complete CACAO 1969

References

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