PMID:12601157

Citation	Bina, J, Zhu, J, Dziejman, M, Faruque, S, Calderwood, S and Mekalanos, J (2003) ToxR regulon of Vibrio cholerae and its expression in vibrios shed by cholera patients. Proc. Natl. Acad. Sci. U.S.A. 100:2801-6
Abstract	Toxigenic Vibrio cholerae cause cholera, a severe diarrheal disease responsible for significant morbidity and mortality worldwide. Two determinants, cholera enterotoxin (CT) and toxin coregulated pilus (TCP) are critical factors responsible for this organism's virulence. The genes for these virulence determinants belong to a network of genes (the ToxR regulon) whose expression is modulated by transcriptional regulators encoded by the toxRS, tcpPH, and toxT genes. To define the ToxR regulon more fully, mutants defective in these regulatory genes were transcriptionally profiled by using V. cholerae genomic microarrays. This study identified 13 genes that were transcriptionally repressed by the toxT mutation (all involved in CT and TCP biogenesis), and 27 and 60 genes that were transcriptionally repressed by the tcpPH and toxRS mutations, respectively. During the course of this analysis, we validated the use of a genomic DNA-based reference sample as a means to standardize and normalize data obtained in different microarray experiments. This method allowed the accurate transcriptional profiling of V. cholerae cells present in stools from cholera patients and the comparison of these profiles to those of wild-type and mutant strains of V. cholerae grown under optimal conditions for CT and TCP expression. Our results suggest that vibrios present in cholera stools carry transcripts for these two virulence determinants, albeit at relatively low levels compared with optimal in vitro conditions. The transcriptional profile of vibrios present in cholera stools also suggests that the bacteria experienced conditions of anaerobiosis, iron limitation, and nutrient deprivation within the human gastrointestinal tract.
Links	PubMed PMC151421 Online version:10.1073/pnas.2628026100
Keywords	Bacterial Proteins; Cholera/metabolism; Cholera/microbiology; DNA, Complementary/metabolism; DNA-Binding Proteins/metabolism; Digestive System/microbiology; Genetic Techniques; Humans; Mutation; Oligonucleotide Array Sequence Analysis; RNA/analysis; RNA, Messenger/metabolism; Transcription Factors/metabolism; Transcription, Genetic; Vibrio cholerae/genetics; Vibrio cholerae/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
VIBCH:TOXR		GO:2000145: regulation of cell motility	ECO:0000270:		P		Table 1. Top right of page 2805	complete CACAO 2217
VIBCH:TOXR		GO:0090087 : regulation of peptide transport	ECO:0000315:		P		Table 1. Top of page 2805 gives more information about specific pathways.	complete CACAO 2220
VIBCH:TOXR		GO:0090087: regulation of peptide transport	ECO:0000270:		P		Table 1. Top of page 2805 gives more information about specific pathways.	complete CACAO 2221
VIBCH:TOXR		GO:0010675: regulation of cellular carbohydrate metabolic process	ECO:0000315:		P		Table 1. Top right of page 2805.	complete CACAO 2222
VIBCH:TOXR		GO:0010675: regulation of cellular carbohydrate metabolic process	ECO:0000270:		P		Table 1. Top right of page 2805.	complete CACAO 2223

References

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