GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:12112233
| Citation |
Thakurta, AG, Ho Yoon, J and Dhar, R (2002) Schizosaccharomyces pombe spPABP, a homologue of Saccharomyces cerevisiae Pab1p, is a non-essential, shuttling protein that facilitates mRNA export. Yeast 19:803-10 |
|---|---|
| Abstract |
Poly(A)-binding proteins play important roles in mRNA metabolism in eukaryotic cells. We examined the role of the Schizosaccharomyces pombe homologue of the Saccharomyces cerevisiae poly(A)-binding protein, Pab1p, in cellular growth and mRNA export. In contrast to PAB1, the sppabp gene is not essential for cellular viability. Like the human hPABP1 protein, spPABP is cytoplasmically localized and can shuttle between the nucleus and the cytoplasm. We found that a spPABP-GFP fusion protein expressed from a multicopy plasmid could suppress the growth and mRNA export defect of rae1-16 7 nup184-1 synthetic lethal mutations. However, about 20-25% of cells in the population exhibited a pronounced nuclear accumulation of poly(A)(+) RNA. The same cells also localized the spPABP-GFP fusion to the nucleus, suggesting that the shuttling ability of spPABP is related to its function in mRNA export. When a heterologous nuclear export activity from spMex67p was fused to spPABP-GFP fusion protein, it overcame the nuclear retention but did not increase nuclear mRNA export. We discuss the implications of these observations in relation to how spPABP could function in mRNA export. Published in 2002 by John Wiley & Sons, Ltd. |
| Links |
PubMed Online version:10.1002/yea.876 |
| Keywords |
Biological Transport; Cell Nucleus/metabolism; Cytoplasm/metabolism; Gene Deletion; Poly(A)-Binding Proteins; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Receptors, Cytoplasmic and Nuclear/metabolism; Schizosaccharomyces/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005634: nucleus |
ECO:0000314: |
C |
complete | |||||
| GO:0005737: cytoplasm |
ECO:0000314: |
C |
complete | |||||
See also
References
See Help:References for how to manage references in GONUTS.
