GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11278069
| Citation |
Wery, M, Woldringh, CL and Rouviere-Yaniv, J (2001) HU-GFP and DAPI co-localize on the Escherichia coli nucleoid. Biochimie 83:193-200 |
|---|---|
| Abstract |
The heterodimeric HU protein, one of the most abundant DNA binding proteins, plays a pleiotropic role in bacteria. Among others, HU was shown to contribute to the maintenance of DNA superhelical density in Escherichia coli. By its properties HU shares some traits with histones and HMG proteins. More recently, its specific binding to DNA recombination and repair intermediates suggests that HU should be considered as a DNA damage sensor. For all these reasons, it will be of interest to follow the localization of HU within the living bacterial cells. To this end, we constructed HU-GFP fusion proteins and compared by microscopy the GFP green fluorescence with images of the nucleoid after DAPI staining. We show that DAPI and HU-GFP colocalize on the E. coli nucleoid. HU, therefore, can be considered as a natural tracer of DNA in the living bacterial cell. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Bacteriolysis; Chromosomes, Bacterial/metabolism; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/metabolism; Fluorescence; Green Fluorescent Proteins; Indoles/metabolism; Luminescent Proteins/metabolism; Plasmids; Recombinant Fusion Proteins/metabolism; Transcription Factors/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:1990178: HU-DNA complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:1990178: HU-DNA complex |
ECO:0000314: |
C |
Fig. 4 |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
