GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:11278069

From GONUTS
Jump to: navigation, search
Citation

Wery, M, Woldringh, CL and Rouviere-Yaniv, J (2001) HU-GFP and DAPI co-localize on the Escherichia coli nucleoid. Biochimie 83:193-200

Abstract

The heterodimeric HU protein, one of the most abundant DNA binding proteins, plays a pleiotropic role in bacteria. Among others, HU was shown to contribute to the maintenance of DNA superhelical density in Escherichia coli. By its properties HU shares some traits with histones and HMG proteins. More recently, its specific binding to DNA recombination and repair intermediates suggests that HU should be considered as a DNA damage sensor. For all these reasons, it will be of interest to follow the localization of HU within the living bacterial cells. To this end, we constructed HU-GFP fusion proteins and compared by microscopy the GFP green fluorescence with images of the nucleoid after DAPI staining. We show that DAPI and HU-GFP colocalize on the E. coli nucleoid. HU, therefore, can be considered as a natural tracer of DNA in the living bacterial cell.

Links

PubMed

Keywords

Bacterial Proteins/metabolism; Bacteriolysis; Chromosomes, Bacterial/metabolism; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/metabolism; Fluorescence; Green Fluorescent Proteins; Indoles/metabolism; Luminescent Proteins/metabolism; Plasmids; Recombinant Fusion Proteins/metabolism; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:DBHA

part_of

GO:1990178: HU-DNA complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:DBHA

GO:1990178: HU-DNA complex

ECO:0000314:

C

Fig. 4

complete
CACAO 8598

See also

References

See Help:References for how to manage references in GONUTS.