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ECOLI:DBHA

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) hupA
Protein Name(s) DNA-binding protein HU-alpha

HU-2 NS2

External Links
UniProt P0ACF0
EMBL X05994
U00006
U00096
AP009048
X54555
D12624
PIR S06269
RefSeq NP_418428.1
YP_491460.1
PDB 1MUL
2O97
PDBsum 1MUL
2O97
ProteinModelPortal P0ACF0
SMR P0ACF0
DIP DIP-35892N
IntAct P0ACF0
MINT MINT-1244407
STRING 511145.b4000
PaxDb P0ACF0
PRIDE P0ACF0
EnsemblBacteria AAC76974
BAE77319
GeneID 12934465
948499
KEGG ecj:Y75_p3196
eco:b4000
PATRIC 32123523
EchoBASE EB0461
EcoGene EG10466
eggNOG COG0776
HOGENOM HOG000043828
InParanoid P0ACF0
KO K05787
OMA MNSITYE
OrthoDB EOG615VS6
PhylomeDB P0ACF0
BioCyc EcoCyc:EG10466-MONOMER
ECOL316407:JW3964-MONOMER
EvolutionaryTrace P0ACF0
PRO PR:P0ACF0
Proteomes UP000000318
UP000000625
Genevestigator P0ACF0
GO GO:0016020
GO:0003677
GO:0042802
GO:0006974
GO:0030261
GO:0006351
Gene3D 4.10.520.10
InterPro IPR000119
IPR020816
IPR010992
Pfam PF00216
PRINTS PR01727
SMART SM00411
SUPFAM SSF47729
PROSITE PS00045

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

part_of

GO:1990178

HU-DNA complex

PMID:11278069[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0016020

membrane

PMID:16858726[2]

ECO:0007005

high throughput direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:21873635[3]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10440
EcoGene:EG10441
EcoGene:EG10466
EcoGene:EG10467
PANTHER:PTN002110664

C

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

PMID:21873635[3]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10466
EcoGene:EG10467
PANTHER:PTN002110658

F

Seeded From UniProt

complete

GO:1990178

HU-DNA complex

PMID:11278069[1]

ECO:0000314

C

Fig. 4

complete
CACAO 8598

enables

GO:0042802

identical protein binding

PMID:12875839[4]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P0ACF0

F

Seeded From UniProt

complete

involved_in

GO:0006974

cellular response to DNA damage stimulus

PMID:11967071[5]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

GO:0003677

DNA binding

PMID:7732012[6]

ECO:0000314

F

Fig 2 graph shows all the mutants and the WT of the Huprotein. When exposed to gamma radiation, the bacteria with the most survivors graphically represented was the WT. RecA13 had the least survivors, with less than 1%. From this data collected, bacteria lacking the HUprotein are much more susceptible to the affects of gamma radiation.

complete

enables

GO:0003677

DNA binding

PMID:10551881[7]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:9218807[8]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

GO:0009432

SOS response

PMID:7732012[6]

ECO:0000314

P

see fig 4

complete

GO:0009650

UV protection

PMID:7732012[6]

ECO:0000314

P

please refer to figure 3 and materials and methods

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:3514923[9]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:227733[10]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:9192630[11]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:11601842[12]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:11254141[13]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

PMID:10982882[14]

ECO:0000270

expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:18304323[15]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000119
InterPro:IPR010992
InterPro:IPR020816

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963

C

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

involved_in

GO:0030261

chromosome condensation

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0226

P

Seeded From UniProt

complete

part_of

GO:0009295

nucleoid

GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-SubCell:SL-0187

C

Seeded From UniProt

complete

edit table

Notes

References

See Help:References for how to manage references in GONUTS.

  1. ↑ 1.0 1.1 Wery, M et al. (2001) HU-GFP and DAPI co-localize on the Escherichia coli nucleoid. Biochimie 83 193-200 PubMed GONUTS page
  2. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed GONUTS page
  3. ↑ 3.0 3.1 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  4. ↑ Ramstein, J et al. (2003) Evidence of a thermal unfolding dimeric intermediate for the Escherichia coli histone-like HU proteins: thermodynamics and structure. J. Mol. Biol. 331 101-21 PubMed GONUTS page
  5. ↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed GONUTS page
  6. ↑ 6.0 6.1 6.2 Boubrik, F & Rouviere-Yaniv, J (1995) Increased sensitivity to gamma irradiation in bacteria lacking protein HU. Proc. Natl. Acad. Sci. U.S.A. 92 3958-62 PubMed GONUTS page
  7. ↑ Azam, TA & Ishihama, A (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J. Biol. Chem. 274 33105-13 PubMed GONUTS page
  8. ↑ Aki, T & Adhya, S (1997) Repressor induced site-specific binding of HU for transcriptional regulation. EMBO J. 16 3666-74 PubMed GONUTS page
  9. ↑ Broyles, SS & Pettijohn, DE (1986) Interaction of the Escherichia coli HU protein with DNA. Evidence for formation of nucleosome-like structures with altered DNA helical pitch. J. Mol. Biol. 187 47-60 PubMed GONUTS page
  10. ↑ Rouvière-Yaniv, J & Kjeldgaard, NO (1979) Native Escherichia coli HU protein is a heterotypic dimer. FEBS Lett. 106 297-300 PubMed GONUTS page
  11. ↑ Painbeni, E et al. (1997) Alterations of the outer membrane composition in Escherichia coli lacking the histone-like protein HU. Proc. Natl. Acad. Sci. U.S.A. 94 6712-7 PubMed GONUTS page
  12. ↑ Delihas, N & Forst, S (2001) MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J. Mol. Biol. 313 1-12 PubMed GONUTS page
  13. ↑ Lee, H et al. (2001) Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli. Mol. Gen. Genet. 264 931-5 PubMed GONUTS page
  14. ↑ Perez, N et al. (2000) A functional assay in Escherichia coli to detect non-assisted interaction between galactose repressor dimers. Nucleic Acids Res. 28 3600-4 PubMed GONUTS page
  15. ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
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