PMID:10684792

Citation	Dong, YL and Yallampalli, C (2000) Pregnancy and exogenous steroid treatments modulate the expression of relaxant EP(2) and contractile FP receptors in the rat uterus. Biol. Reprod. 62:533-9
Abstract	Prostaglandins (PGs) interact with specific receptors on plasma membranes to regulate myometrial activity in many species. The present study examined whether the expression of relaxant prostaglandin E receptor subtype two (EP(2)) and contractile prostaglandin F receptor (FP) mRNA in the rat uterus is changed during various states of pregnancy and regulated by steroid hormones. Expression of mRNA for EP(2) and FP receptors in the full-thickness uteri was analyzed by reverse transcription-polymerase chain reaction using specific primers. Abundance of receptor mRNA was expressed relative to beta-actin mRNA. Results showed that 1) mRNA for EP(2) receptors in the rat uterus was substantially increased during pregnancy (320%) compared with the nonpregnant state (100%, P < 0.01), and declined during labor at term (36% vs. 100% in control, P < 0.01); 2) mRNA expression for FP receptors in rat uterus was increased during pregnancy (333% vs. 100% in nonpregnant rats, P < 0. 01) and reached maximal levels during labor (515% vs. 100% in control, P < 0.01); 3) upon RU-486 treatment on Day 19 of pregnancy, uterine EP(2) receptor mRNA levels were decreased (18% vs. 100% in control, P < 0.01), and FP mRNA levels were increased (357% vs. 100% in control, P < 0.01); 4) with ICI 164384 (an antiestrogen) treatment on Day 19 of gestation, uterine FP receptor mRNA levels were decreased without effects on EP(2) receptors; 5) in ovariectomized (ovx) rats, progesterone increased EP(2) (163% vs. 100% in control, P < 0.01) and had no effects on FP receptor mRNA expression in the rat uterus; 6) estradiol increased FP receptor mRNA levels (358% vs. 100% in control, P < 0.01) and had no effects on EP(2) mRNA in the ovx rat uterus. Therefore, we conclude that steroid hormones modulate the mRNA for relaxant EP(2) and contractile FP receptors for PGs in the uterus and thus regulate uterine activity during pregnancy and labor.
Links	PubMed
Keywords	Animals; Estradiol/analogs & derivatives; Estradiol/metabolism; Estradiol/pharmacology; Estrogen Antagonists/pharmacology; Female; Gene Expression Regulation; Hormone Antagonists/pharmacology; Labor, Obstetric/metabolism; Mifepristone/pharmacology; Polyunsaturated Alkamides; Postpartum Period/metabolism; Pregnancy; Pregnancy, Animal/metabolism; Progesterone/metabolism; Progesterone/pharmacology; Rats; Rats, Sprague-Dawley; Receptors, Prostaglandin/drug effects; Receptors, Prostaglandin/genetics; Receptors, Prostaglandin E/drug effects; Receptors, Prostaglandin E/genetics; Receptors, Prostaglandin E, EP2 Subtype; Steroids/metabolism; Steroids/pharmacology; Uterine Contraction/drug effects; Uterine Contraction/metabolism; Uterus/drug effects; Uterus/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:PE2R2		GO:0032570: response to progesterone stimulus	ECO:0000314:		P		Positive regulation shown in figure 4	complete CACAO 2178
MOUSE:PE2R2	involved_in	GO:0032570: response to progesterone	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:PF2R	involved_in	GO:0032355: response to estradiol	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
MOUSE:PF2R		GO:0032355: response to estradiol stimulus	ECO:0000314:		P		Positive regulation to estradiol-17β from figure 7	complete CACAO 2195

References

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