PMID:10100151

Citation	Martineau, LC, Chadan, SG and Parkhouse, WS (1999) Age-associated alterations in cardiac and skeletal muscle glucose transporters, insulin and IGF-1 receptors, and PI3-kinase protein contents in the C57BL/6 mouse. Mech. Ageing Dev. 106:217-32
Abstract	We investigated potential age-related changes in cardiac and skeletal muscle protein contents of glut-4 and glut-1 transporters, insulin and insulin-like growth factor-1 (IGF-1) receptors, and phosphatidylinositol 3-kinase (PI3-kinase) in the C57B1/6 mouse. Myocardial glut-4 content increased four- to five-fold between mid- to late-adulthood with no further age-related changes. Increases in myocardial glut-1 preceded the increase in glut-4 and was of a much smaller magnitude (25-40%). Skeletal muscle glut-4 was also increased (38-49%) and no further changes were noted between adulthood and old age. Cardiac insulin receptor and the p85 alpha subunit of PI3-kinase both declined by about 40%, whereas the skeletal muscle content of these two proteins were unaffected by aging. Cardiac (-23 to -24%) and skeletal muscle (-40 to -62%) IGF-1 receptor levels were decreased in adult and old animals with senescence being associated with a further decrease in cardiac IGF-1 receptor levels to 20% of controls. A two- to three-fold increase in both basal and maximal in vitro autophosphorylation of the cardiac insulin and IGF-1 receptors by their respective ligands was observed with senescence. It appears that cardiac and skeletal muscle demonstrate differential responses in terms of the magnitude and temporal responses of age-associated alterations in glucose transport related protein contents in the C57B1/6 mouse.
Links	PubMed
Keywords	Aging/metabolism; Animals; Biomarkers; Blotting, Western; Female; Follow-Up Studies; Heart/growth & development; Mice; Mice, Inbred C57BL; Monosaccharide Transport Proteins/metabolism; Muscle Development; Muscle, Skeletal/growth & development; Muscle, Skeletal/metabolism; Myocardium/metabolism; Phosphatidylinositol 3-Kinases/metabolism; Phosphorylation; Receptor, IGF Type 1/metabolism; Receptor, Insulin/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
MOUSE:IGF1R	acts_upstream_of_or_within	GO:0038083: peptidyl-tyrosine autophosphorylation	ECO:0000314: direct assay evidence used in manual assertion		P	occurs_in:(EMAPA:16105)	Seeded From UniProt	complete
MOUSE:INSR	acts_upstream_of_or_within	GO:0038083: peptidyl-tyrosine autophosphorylation	ECO:0000314: direct assay evidence used in manual assertion		P	occurs_in:(EMAPA:16105)	Seeded From UniProt	complete

References

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