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HUMAN:HIF1N
Contents
| Species (Taxon ID) | Homo sapiens (Human). (9606) | |
| Gene Name(s) | HIF1AN (synonyms: FIH1) | |
| Protein Name(s) | Hypoxia-inducible factor 1-alpha inhibitor
Factor inhibiting HIF-1 FIH-1 Hypoxia-inducible factor asparagine hydroxylase | |
| External Links | ||
| UniProt | Q9NWT6 | |
| EMBL | AF395830 AK000622 AL133352 CH471066 CH471066 BC007719 AL359615 | |
| CCDS | CCDS7498.1 | |
| PIR | T50633 | |
| RefSeq | NP_060372.2 | |
| UniGene | Hs.500788 | |
| PDB | 1H2K 1H2L 1H2M 1H2N 1IZ3 1MZE 1MZF 1YCI 2CGN 2CGO 2ILM 2W0X 2WA3 2WA4 2XUM 2Y0I 2YC0 2YDE 3D8C 3KCX 3KCY 3OD4 3P3N 3P3P 4AI8 4B7E 4B7K 4BIO 4JAA | |
| PDBsum | 1H2K 1H2L 1H2M 1H2N 1IZ3 1MZE 1MZF 1YCI 2CGN 2CGO 2ILM 2W0X 2WA3 2WA4 2XUM 2Y0I 2YC0 2YDE 3D8C 3KCX 3KCY 3OD4 3P3N 3P3P 4AI8 4B7E 4B7K 4BIO 4JAA | |
| ProteinModelPortal | Q9NWT6 | |
| SMR | Q9NWT6 | |
| BioGrid | 120794 | |
| IntAct | Q9NWT6 | |
| MINT | MINT-1465958 | |
| STRING | 9606.ENSP00000299163 | |
| BindingDB | Q9NWT6 | |
| ChEMBL | CHEMBL5909 | |
| PhosphoSite | Q9NWT6 | |
| DMDM | 32129605 | |
| MaxQB | Q9NWT6 | |
| PaxDb | Q9NWT6 | |
| PRIDE | Q9NWT6 | |
| DNASU | 55662 | |
| Ensembl | ENST00000299163 | |
| GeneID | 55662 | |
| KEGG | hsa:55662 | |
| UCSC | uc001krj.4 | |
| CTD | 55662 | |
| GeneCards | GC10P102285 | |
| HGNC | HGNC:17113 | |
| HPA | HPA048742 | |
| MIM | 606615 | |
| neXtProt | NX_Q9NWT6 | |
| PharmGKB | PA29284 | |
| eggNOG | NOG71927 | |
| GeneTree | ENSGT00530000062914 | |
| HOGENOM | HOG000008146 | |
| HOVERGEN | HBG051903 | |
| InParanoid | Q9NWT6 | |
| KO | K18055 | |
| OMA | MIKGRYD | |
| PhylomeDB | Q9NWT6 | |
| TreeFam | TF329609 | |
| BioCyc | MetaCyc:HS15407-MONOMER | |
| Reactome | REACT_121226 | |
| ChiTaRS | HIF1AN | |
| EvolutionaryTrace | Q9NWT6 | |
| GeneWiki | HIF1AN | |
| GenomeRNAi | 55662 | |
| NextBio | 60403 | |
| PRO | PR:Q9NWT6 | |
| Proteomes | UP000005640 | |
| Bgee | Q9NWT6 | |
| CleanEx | HS_HIF1AN | |
| ExpressionAtlas | Q9NWT6 | |
| Genevestigator | Q9NWT6 | |
| GO | GO:0005737 GO:0005829 GO:0005634 GO:0048471 GO:0071532 GO:0031406 GO:0048037 GO:0005506 GO:0051059 GO:0005112 GO:0016706 GO:0019826 GO:0036140 GO:0036139 GO:0042803 GO:0008270 GO:0071456 GO:0045746 GO:0061428 GO:0055114 GO:0042265 GO:0042264 GO:0036138 GO:0045663 GO:2001214 GO:0061418 GO:0006351 | |
| Gene3D | 1.10.287.1010 | |
| InterPro | IPR027445 IPR027452 IPR003347 | |
| PANTHER | PTHR12461:SF19 | |
| SMART | SM00558 | |
| PROSITE | PS51184 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0018126 |
protein hydroxylation |
ECO:0000314 |
P |
Figure 1 shows the effect of overexpression of FIH. This results in the hydroxylation of Asn. |
complete | |||||
| GO:0043433 |
negative regulation of sequence-specific DNA binding transcription factor activity |
ECO:0000315 |
P |
Figure 3 |
complete | |||||
| GO:0008593 |
regulation of Notch signaling pathway |
ECO:0000315 |
P |
Figure 1: To control the Notch downstream response, FIH-1 hydroxylates N1945 and N2012 of Notch 1 ICD, thereby regulating Notch activity. |
complete | |||||
| GO:0030528 |
transcription regulator activity |
ECO:0000314 |
F |
Figure 3: FIH-1 inhibits the transcriptional activity of HIF-1alpha. |
complete | |||||
|
involved_in |
GO:2001214 |
positive regulation of vasculogenesis |
ECO:0000303 |
author statement without traceable support used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0061428 |
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051059 |
NF-kappaB binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0048471 |
perinuclear region of cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0048037 |
cofactor binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045746 |
negative regulation of Notch signaling pathway |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045663 |
positive regulation of myoblast differentiation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042803 |
protein homodimerization activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0042264 |
peptidyl-aspartic acid hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0036140 |
peptidyl-asparagine 3-dioxygenase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
has_direct_input:(UniProtKB:O14974) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0036140 |
peptidyl-asparagine 3-dioxygenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0036140 |
peptidyl-asparagine 3-dioxygenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0036139 |
peptidyl-histidine dioxygenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0036138 |
peptidyl-histidine hydroxylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0031406 |
carboxylic acid binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0019826 |
oxygen sensor activity |
ECO:0000303 |
author statement without traceable support used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008270 |
zinc ion binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
colocalizes_with |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005506 |
iron ion binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005112 |
Notch binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005112 |
Notch binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005112 |
Notch binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0071532 |
ankyrin repeat binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0061428 |
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0045746 |
negative regulation of Notch signaling pathway |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0042265 |
peptidyl-asparagine hydroxylation |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0042264 |
peptidyl-aspartic acid hydroxylation |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0036140 |
peptidyl-asparagine 3-dioxygenase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0036139 |
peptidyl-histidine dioxygenase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0036138 |
peptidyl-histidine hydroxylation |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016706 |
2-oxoglutarate-dependent dioxygenase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN002488274 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000967060 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005654 |
nucleoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0102113 |
hypoxia-inducible factor-asparagine oxygenase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0061418 |
regulation of transcription from RNA polymerase II promoter in response to hypoxia |
Reactome:R-HSA-1234174 |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005829 |
cytosol |
Reactome:R-HSA-1234164 |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0051213 |
dioxygenase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0048471 |
perinuclear region of cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Dayan, F et al. (2006) The oxygen sensor factor-inhibiting hypoxia-inducible factor-1 controls expression of distinct genes through the bifunctional transcriptional character of hypoxia-inducible factor-1alpha. Cancer Res. 66 3688-98 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Mahon, PC et al. (2001) FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity. Genes Dev. 15 2675-86 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 3.4 Zheng, X et al. (2008) Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. Proc. Natl. Acad. Sci. U.S.A. 105 3368-73 PubMed GONUTS page
- ↑ 4.0 4.1 Ferguson, JE 3rd et al. (2007) ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism. Mol. Cell. Biol. 27 6407-19 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 Yang, M et al. (2011) Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains. FEBS J. 278 1086-97 PubMed GONUTS page
- ↑ 6.0 6.1 6.2 6.3 Yang, M et al. (2011) Asparagine and aspartate hydroxylation of the cytoskeletal ankyrin family is catalyzed by factor-inhibiting hypoxia-inducible factor. J. Biol. Chem. 286 7648-60 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 7.3 Cockman, ME et al. (2006) Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Proc. Natl. Acad. Sci. U.S.A. 103 14767-72 PubMed GONUTS page
- ↑ 8.0 8.1 8.2 McNeill, LA et al. (2002) Hypoxia-inducible factor asparaginyl hydroxylase (FIH-1) catalyses hydroxylation at the beta-carbon of asparagine-803. Biochem. J. 367 571-5 PubMed GONUTS page
- ↑ 9.0 9.1 9.2 Lando, D et al. (2002) FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor. Genes Dev. 16 1466-71 PubMed GONUTS page
- ↑ Sakamoto, T & Seiki, M (2009) Mint3 enhances the activity of hypoxia-inducible factor-1 (HIF-1) in macrophages by suppressing the activity of factor inhibiting HIF-1. J. Biol. Chem. 284 30350-9 PubMed GONUTS page
- ↑ 11.0 11.1 11.2 11.3 Elkins, JM et al. (2003) Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha. J. Biol. Chem. 278 1802-6 PubMed GONUTS page
- ↑ 12.0 12.1 Webb, JD et al. (2009) MYPT1, the targeting subunit of smooth-muscle myosin phosphatase, is a substrate for the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH). Biochem. J. 420 327-33 PubMed GONUTS page
- ↑ 13.0 13.1 13.2 Linke, S et al. (2004) Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor. J. Biol. Chem. 279 14391-7 PubMed GONUTS page
- ↑ Hewitson, KS et al. (2007) Structural and mechanistic studies on the inhibition of the hypoxia-inducible transcription factor hydroxylases by tricarboxylic acid cycle intermediates. J. Biol. Chem. 282 3293-301 PubMed GONUTS page
- ↑ 15.0 15.1 Coleman, ML et al. (2007) Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor. J. Biol. Chem. 282 24027-38 PubMed GONUTS page
- ↑ 16.00 16.01 16.02 16.03 16.04 16.05 16.06 16.07 16.08 16.09 16.10 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
c
h
n
o
- GO:0048037 ! obsolete cofactor binding
- GO:0061428 ! obsolete negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
- GO:0055114 ! obsolete oxidation-reduction process
- GO:0036138 ! obsolete peptidyl-histidine hydroxylation
- GO:0042265 ! obsolete peptidyl-asparagine hydroxylation
- GO:0061418 ! obsolete regulation of transcription from RNA polymerase II promoter in response to hypoxia
- GO:0030528 ! obsolete transcription regulator activity
- GO:0016491 ! oxidoreductase activity
- GO:0019826 ! oxygen sensor activity
p
- GO:0042264 ! peptidyl-aspartic acid hydroxylation
- GO:0036139 ! peptidyl-histidine dioxygenase activity
- GO:0048471 ! perinuclear region of cytoplasm
- GO:2001214 ! positive regulation of vasculogenesis
- GO:0045663 ! positive regulation of myoblast differentiation
- Primates
- GO:0042803 ! protein homodimerization activity
- GO:0018126 ! protein hydroxylation
