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ECOLI:PUTA
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | putA (synonyms: poaA) | |
| Protein Name(s) | Bifunctional protein PutA
Proline dehydrogenase Proline oxidase Delta-1-pyrroline-5-carboxylate dehydrogenase P5C dehydrogenase L-glutamate gamma-semialdehyde dehydrogenase | |
| External Links | ||
| UniProt | P09546 | |
| EMBL | U05212 U00096 AP009048 X05653 | |
| PIR | D64843 | |
| RefSeq | NP_415534.1 YP_489287.1 | |
| PDB | 1TIW 1TJ0 1TJ1 1TJ2 2AY0 2FZM 2FZN 2GPE 2RBF 3E2Q 3E2R 3E2S 3ITG 4JNY 4JNZ 4O8A | |
| PDBsum | 1TIW 1TJ0 1TJ1 1TJ2 2AY0 2FZM 2FZN 2GPE 2RBF 3E2Q 3E2R 3E2S 3ITG 4JNY 4JNZ 4O8A | |
| ProteinModelPortal | P09546 | |
| SMR | P09546 | |
| DIP | DIP-10620N | |
| IntAct | P09546 | |
| MINT | MINT-1267731 | |
| STRING | 511145.b1014 | |
| DrugBank | DB03147 | |
| PaxDb | P09546 | |
| PRIDE | P09546 | |
| EnsemblBacteria | AAC74099 BAA35791 | |
| GeneID | 12933852 945600 | |
| KEGG | ecj:Y75_p0987 eco:b1014 | |
| PATRIC | 32117257 | |
| EchoBASE | EB0794 | |
| EcoGene | EG10801 | |
| eggNOG | COG0506 | |
| HOGENOM | HOG000253911 | |
| InParanoid | P09546 | |
| KO | K13821 | |
| OMA | PILHVVR | |
| OrthoDB | EOG6BS8QW | |
| PhylomeDB | P09546 | |
| BioCyc | EcoCyc:PUTA-MONOMER ECOL316407:JW0999-MONOMER MetaCyc:PUTA-MONOMER RETL1328306-WGS:GSTH-4764-MONOMER | |
| UniPathway | UPA00261 UPA00261 | |
| EvolutionaryTrace | P09546 | |
| PRO | PR:P09546 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P09546 | |
| GO | GO:0009898 GO:0003842 GO:0000986 GO:0001141 GO:0003677 GO:0050660 GO:0016620 GO:0004657 GO:0043565 GO:0045892 GO:0006561 GO:0010133 | |
| Gene3D | 1.10.2060.10 1.20.5.550 3.20.20.220 3.40.309.10 3.40.605.10 | |
| InterPro | IPR016161 IPR016163 IPR016160 IPR029510 IPR016162 IPR015590 IPR025703 IPR005933 IPR029041 IPR024090 IPR024089 IPR024082 IPR002872 IPR010985 | |
| Pfam | PF00171 PF01619 PF14850 | |
| PIRSF | PIRSF000197 | |
| SUPFAM | SSF47598 SSF51730 SSF53720 SSF81935 | |
| TIGRFAMs | TIGR01238 | |
| PROSITE | PS00070 PS00687 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0004657 |
proline dehydrogenase activity |
ECO:0000314 |
F |
Table 3 and Figure 3 both show that proline dehydrogenase activity happens here |
complete | |||||
| GO:0016020 |
membrane |
ECO:0000315 |
C |
Figure 4 |
complete | |||||
| GO:0004657 |
proline dehydrogenase activity |
ECO:0000315 |
F |
Figure 2 |
complete | |||||
|
enables |
GO:0004657 |
proline dehydrogenase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004657 |
proline dehydrogenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0043565 |
sequence-specific DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004657 |
proline dehydrogenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010133 |
proline catabolic process to glutamate |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10801 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0009898 |
cytoplasmic side of plasma membrane |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10801 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0006560 |
proline metabolic process |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10801 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003842 |
1-pyrroline-5-carboxylate dehydrogenase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG10801 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0050660 |
flavin adenine dinucleotide binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010133 |
proline catabolic process to glutamate |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0009898 |
cytoplasmic side of plasma membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004657 |
proline dehydrogenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003842 |
1-pyrroline-5-carboxylate dehydrogenase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0001217 |
DNA-binding transcription repressor activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000986 |
bacterial-type proximal promoter sequence-specific DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045892 |
negative regulation of transcription, DNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0001217 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003700 |
DNA-binding transcription factor activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003842 |
1-pyrroline-5-carboxylate dehydrogenase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR005933 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0004657 |
proline dehydrogenase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006355 |
regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006561 |
proline biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010133 |
proline catabolic process to glutamate |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR015590 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016620 |
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
InterPro:IPR005933 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0055114 |
oxidation-reduction process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008152 |
metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016491 |
oxidoreductase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006560 |
proline metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010133 |
proline catabolic process to glutamate |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniPathway:UPA00261 |
P |
Seeded From UniProt |
complete | ||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Moxley, MA et al. (2011) Steady-state kinetic mechanism of the proline:ubiquinone oxidoreductase activity of proline utilization A (PutA) from Escherichia coli. Arch. Biochem. Biophys. 516 113-20 PubMed GONUTS page
- ↑ Zhou, Y et al. (2008) Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli. Amino Acids 35 711-8 PubMed GONUTS page
- ↑ 3.0 3.1 Ostrander, EL et al. (2009) A conserved active site tyrosine residue of proline dehydrogenase helps enforce the preference for proline over hydroxyproline as the substrate. Biochemistry 48 951-9 PubMed GONUTS page
- ↑ Gu, D et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. J. Biol. Chem. 279 31171-6 PubMed GONUTS page
- ↑ Graham, SB et al. (1984) Proline dehydrogenase from Escherichia coli K12. Reconstitution of a functional membrane association. J. Biol. Chem. 259 2656-61 PubMed GONUTS page
- ↑ 6.0 6.1 6.2 6.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Brown, ED & Wood, JM (1992) Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli. J. Biol. Chem. 267 13086-92 PubMed GONUTS page
- ↑ Lee, YH et al. (2003) Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein. Nat. Struct. Biol. 10 109-14 PubMed GONUTS page
- ↑ Vinod, MP et al. (2002) Electrochemical and functional characterization of the proline dehydrogenase domain of the PutA flavoprotein from Escherichia coli. Biochemistry 41 6525-32 PubMed GONUTS page
- ↑ Wood, JM & Zadworny, D (1980) Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can. J. Biochem. 58 787-96 PubMed GONUTS page
- ↑ 11.0 11.1 Zhou, Y et al. (2008) Structural basis of the transcriptional regulation of the proline utilization regulon by multifunctional PutA. J. Mol. Biol. 381 174-88 PubMed GONUTS page
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