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PMID:18324349
| Citation |
Zhou, Y, Zhu, W, Bellur, PS, Rewinkel, D and Becker, DF (2008) Direct linking of metabolism and gene expression in the proline utilization A protein from Escherichia coli. Amino Acids 35:711-8 |
|---|---|
| Abstract |
The control of gene expression by enzymes provides a direct pathway for cells to respond to fluctuations in metabolites and nutrients. One example is the proline utilization A (PutA) protein from Escherichia coli. PutA is a membrane-associated enzyme that catalyzes the oxidation of L: -proline to glutamate using a flavin containing proline dehydrogenase domain and a NAD(+) dependent Delta(1)-pyrroline-5-carboxylate dehydrogenase domain. In some Gram-negative bacteria such as E. coli, PutA is also endowed with a ribbon-helix-helix DNA-binding domain and acts as a transcriptional repressor of the proline utilization genes. PutA switches between transcriptional repressor and enzymatic functions in response to proline availability. Molecular insights into the redox-based mechanism of PutA functional switching from recent studies are reviewed. In addition, new results from cell-based transcription assays are presented which correlate PutA membrane localization with put gene expression levels. General membrane localization of PutA, however, is not sufficient to activate the put genes. |
| Links |
PubMed PMC2666929 Online version:10.1007/s00726-008-0053-6 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/physiology; Cell Membrane/metabolism; DNA/chemistry; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Glutamic Acid/chemistry; Kinetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Proteins/physiology; Models, Biological; Oxidation-Reduction; Oxygen/chemistry; Proline/chemistry; Protein Conformation; Protein Structure, Tertiary; Surface Plasmon Resonance; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0016020: membrane |
ECO:0000315: |
C |
Figure 4 |
complete | ||||
See also
References
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