ECOLI:PBP2

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	mrdA (synonyms: pbpA)
Protein Name(s)	Penicillin-binding protein 2 PBP-2
External Links
UniProt	P0AD65
EMBL	X04516 U82598 U00096 AP009048 M22857
PIR	C24995
RefSeq	NP_415168.1 YP_488926.1
ProteinModelPortal	P0AD65
SMR	P0AD65
DIP	DIP-48190N
IntAct	P0AD65
STRING	511145.b0635
ChEMBL	CHEMBL2354204
DrugBank	DB01327 DB01413 DB01328 DB01329 DB00438 DB01415 DB00303 DB01598 DB00948
PaxDb	P0AD65
PRIDE	P0AD65
EnsemblBacteria	AAC73736 BAA35282
GeneID	12930918 945240
KEGG	ecj:Y75_p0625 eco:b0635
PATRIC	32116453
EchoBASE	EB0601
EcoGene	EG10606
eggNOG	COG0768
HOGENOM	HOG000266120
InParanoid	P0AD65
KO	K05515
OMA	ARNYSAY
OrthoDB	EOG6N0HHV
PhylomeDB	P0AD65
BioCyc	EcoCyc:EG10606-MONOMER ECOL316407:JW0630-MONOMER MetaCyc:EG10606-MONOMER
UniPathway	UPA00219
PRO	PR:P0AD65
Proteomes	UP000000318 UP000000625
Genevestigator	P0AD65
GO	GO:0005887 GO:0008144 GO:0008658 GO:0071972 GO:0009002 GO:0071555 GO:0009252 GO:0006508 GO:0008360 GO:0046677 GO:0042493
Gene3D	3.40.710.10
InterPro	IPR012338 IPR005311 IPR001460 IPR017790
Pfam	PF03717 PF00905
SUPFAM	SSF56519 SSF56601
TIGRFAMs	TIGR03423

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0009252	peptidoglycan biosynthetic process	PMID:PMC2681889	ECO:0000315			P	figure 7 shows penicilin binding protein 2 untethers the incorporation of peptidoglycan in the cell walls	complete CACAO 5129
enables	GO:0071972	peptidoglycan L,D-transpeptidase activity	PMID:21873635^[1]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10606 PANTHER:PTN000771407	F	Seeded From UniProt	complete
involved_in	GO:0071555	cell wall organization	PMID:21873635^[1]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10606 PANTHER:PTN000771406	P	Seeded From UniProt	complete
enables	GO:0008658	penicillin binding	PMID:21873635^[1]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10341 EcoGene:EG10606 PANTHER:PTN000771406	F	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:21873635^[1]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10341 EcoGene:EG10606 PANTHER:PTN000771406	C	Seeded From UniProt	complete
enables	GO:0071972	peptidoglycan L,D-transpeptidase activity	PMID:6297485^[2]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0046677	response to antibiotic	PMID:1103132^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0008658	penicillin binding	PMID:1103132^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:1103132^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:1103132^[3]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0071555	cell wall organization	PMID:3326629^[4]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0071555	cell wall organization	PMID:18390656^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0071555	cell wall organization	PMID:11418550^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0042493	response to drug	PMID:18390656^[5]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0042493	response to drug	PMID:18250190^[7]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0042493	response to drug	PMID:17470659^[8]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009252	peptidoglycan biosynthetic process	PMID:8760906^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009252	peptidoglycan biosynthetic process	PMID:18390656^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	PMID:1731901^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	PMID:3009484^[11]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:3894330^[12]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:18390656^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:17513478^[13]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:12519203^[14]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	PMID:11418550^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0008144	drug binding	PMID:18250190^[7]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008144	drug binding	PMID:17470659^[8]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008144	drug binding	PMID:12886052^[15]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008144	drug binding	PMID:12709336^[16]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008658	penicillin binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001460 InterPro:IPR005311 InterPro:IPR017790 InterPro:IPR036138	F	Seeded From UniProt	complete
enables	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR017790	F	Seeded From UniProt	complete
involved_in	GO:0009252	peptidoglycan biosynthetic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR017790	P	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR017790	C	Seeded From UniProt	complete
enables	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.4.16.4	F	Seeded From UniProt	complete
enables	GO:0009002	serine-type D-Ala-D-Ala carboxypeptidase activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000432224	F	Seeded From UniProt	complete
involved_in	GO:0009252	peptidoglycan biosynthetic process	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000432224	P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000432224	C	Seeded From UniProt	complete
involved_in	GO:0006508	proteolysis	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0645	P	Seeded From UniProt	complete
involved_in	GO:0009252	peptidoglycan biosynthetic process	GO_REF:0000037 GO_REF:0000041	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0573 UniPathway:UPA00219	P	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000037 GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1003 UniProtKB-KW:KW-0997 UniProtKB-SubCell:SL-0037	C	Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C	Seeded From UniProt	complete
enables	GO:0004180	carboxypeptidase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0121	F	Seeded From UniProt	complete
involved_in	GO:0071555	cell wall organization	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0961	P	Seeded From UniProt	complete
involved_in	GO:0008360	regulation of cell shape	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0133	P	Seeded From UniProt	complete
enables	GO:0008233	peptidase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0645	F	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ Ishino, F et al. (1982) A mecillinam-sensitive peptidoglycan crosslinking reaction in Escherichia coli. Biochem. Biophys. Res. Commun. 109 689-96 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 Spratt, BG (1975) Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc. Natl. Acad. Sci. U.S.A. 72 2999-3003 PubMed GONUTS page
↑ Buchnik, D et al. () Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli. Ann. Inst. Pasteur Microbiol. 138 537-47 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 Uehara, T & Park, JT (2008) Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation. J. Bacteriol. 190 3914-22 PubMed GONUTS page
↑ ^6.0 ^6.1 de Pedro, MA et al. (2001) Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2. J. Bacteriol. 183 4115-26 PubMed GONUTS page
↑ ^7.0 ^7.1 Davies, TA et al. (2008) Affinity of doripenem and comparators to penicillin-binding proteins in Escherichia coli and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52 1510-2 PubMed GONUTS page
↑ ^8.0 ^8.1 Davies, TA et al. (2007) Binding of ceftobiprole and comparators to the penicillin-binding proteins of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Antimicrob. Agents Chemother. 51 2621-4 PubMed GONUTS page
↑ Signoretto, C et al. (1996) Modified peptidoglycan chemical composition in shape-altered Escherichia coli. Microbiology (Reading, Engl.) 142 ( Pt 8) 1919-26 PubMed GONUTS page
↑ Adachi, H et al. (1992) Active-site residues of the transpeptidase domain of penicillin-binding protein 2 from Escherichia coli: similarity in catalytic mechanism to class A beta-lactamases. Biochemistry 31 430-7 PubMed GONUTS page
↑ Ishino, F et al. (1986) Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein. J. Biol. Chem. 261 7024-31 PubMed GONUTS page
↑ Begg, KJ & Donachie, WD (1985) Cell shape and division in Escherichia coli: experiments with shape and division mutants. J. Bacteriol. 163 615-22 PubMed GONUTS page
↑ Legaree, BA et al. (2007) Overproduction of penicillin-binding protein 2 and its inactive variants causes morphological changes and lysis in Escherichia coli. J. Bacteriol. 189 4975-83 PubMed GONUTS page
↑ Den Blaauwen, T et al. (2003) Penicillin-binding protein PBP2 of Escherichia coli localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole. Mol. Microbiol. 47 539-47 PubMed GONUTS page
↑ Dalhoff, A et al. (2003) Target affinities of faropenem to and its impact on the morphology of gram-positive and gram-negative bacteria. Chemotherapy 49 172-83 PubMed GONUTS page
↑ Jamieson, CE et al. (2003) In vitro and in vivo activities of AM-112, a novel oxapenem. Antimicrob. Agents Chemother. 47 1652-7 PubMed GONUTS page

[PMID:21873635-1] 1.0 ^1.1 ^1.2 ^1.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:6297485-2] Ishino, F et al. (1982) A mecillinam-sensitive peptidoglycan crosslinking reaction in Escherichia coli. Biochem. Biophys. Res. Commun. 109 689-96 PubMed GONUTS page

[PMID:1103132-3] 3.0 ^3.1 ^3.2 ^3.3 Spratt, BG (1975) Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc. Natl. Acad. Sci. U.S.A. 72 2999-3003 PubMed GONUTS page

[PMID:3326629-4] Buchnik, D et al. () Effects of temperature inactivation of penicillin-binding protein 2 on envelope growth in Escherichia coli. Ann. Inst. Pasteur Microbiol. 138 537-47 PubMed GONUTS page

[PMID:18390656-5] 5.0 ^5.1 ^5.2 ^5.3 Uehara, T & Park, JT (2008) Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation. J. Bacteriol. 190 3914-22 PubMed GONUTS page

[PMID:11418550-6] 6.0 ^6.1 de Pedro, MA et al. (2001) Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2. J. Bacteriol. 183 4115-26 PubMed GONUTS page

[PMID:18250190-7] 7.0 ^7.1 Davies, TA et al. (2008) Affinity of doripenem and comparators to penicillin-binding proteins in Escherichia coli and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52 1510-2 PubMed GONUTS page

[PMID:17470659-8] 8.0 ^8.1 Davies, TA et al. (2007) Binding of ceftobiprole and comparators to the penicillin-binding proteins of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Antimicrob. Agents Chemother. 51 2621-4 PubMed GONUTS page

[PMID:8760906-9] Signoretto, C et al. (1996) Modified peptidoglycan chemical composition in shape-altered Escherichia coli. Microbiology (Reading, Engl.) 142 ( Pt 8) 1919-26 PubMed GONUTS page

[PMID:1731901-10] Adachi, H et al. (1992) Active-site residues of the transpeptidase domain of penicillin-binding protein 2 from Escherichia coli: similarity in catalytic mechanism to class A beta-lactamases. Biochemistry 31 430-7 PubMed GONUTS page

[PMID:3009484-11] Ishino, F et al. (1986) Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein. J. Biol. Chem. 261 7024-31 PubMed GONUTS page

[PMID:3894330-12] Begg, KJ & Donachie, WD (1985) Cell shape and division in Escherichia coli: experiments with shape and division mutants. J. Bacteriol. 163 615-22 PubMed GONUTS page

[PMID:17513478-13] Legaree, BA et al. (2007) Overproduction of penicillin-binding protein 2 and its inactive variants causes morphological changes and lysis in Escherichia coli. J. Bacteriol. 189 4975-83 PubMed GONUTS page

[PMID:12519203-14] Den Blaauwen, T et al. (2003) Penicillin-binding protein PBP2 of Escherichia coli localizes preferentially in the lateral wall and at mid-cell in comparison with the old cell pole. Mol. Microbiol. 47 539-47 PubMed GONUTS page

[PMID:12886052-15] Dalhoff, A et al. (2003) Target affinities of faropenem to and its impact on the morphology of gram-positive and gram-negative bacteria. Chemotherapy 49 172-83 PubMed GONUTS page

[PMID:12709336-16] Jamieson, CE et al. (2003) In vitro and in vivo activities of AM-112, a novel oxapenem. Antimicrob. Agents Chemother. 47 1652-7 PubMed GONUTS page

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ECOLI:PBP2

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