ECOLI:EMRE

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	emrE (synonyms: eb, mvrC)
Protein Name(s)	Multidrug transporter EmrE Efflux-multidrug resistance protein EmrE Ethidium resistance protein Methyl viologen resistance protein C
External Links
UniProt	P23895
EMBL	Z11877 M62732 U82598 U00096 AP009048
PIR	JN0329
RefSeq	NP_415075.1 YP_488830.1
PDB	2I68 3B5D 3B61 3B62
PDBsum	2I68 3B5D 3B61 3B62
ProteinModelPortal	P23895
DIP	DIP-9505N
STRING	511145.b0543
TCDB	2.A.7.1.3
DNASU	948442
EnsemblBacteria	AAC73644 BAE76318
GeneID	12930372 948442
KEGG	ecj:Y75_p0528 eco:b0543
PATRIC	32116248
EchoBASE	EB0623
EcoGene	EG10629
eggNOG	COG2076
HOGENOM	HOG000268006
InParanoid	P23895
KO	K03297
OMA	GVVYGTW
OrthoDB	EOG69WFSW
PhylomeDB	P23895
BioCyc	EcoCyc:EMRE-MONOMER ECOL316407:JW0531-MONOMER MetaCyc:EMRE-MONOMER RETL1328306-WGS:GSTH-3313-MONOMER
SABIO-RK	P23895
EvolutionaryTrace	P23895
PRO	PR:P23895
Proteomes	UP000000318 UP000000625
Genevestigator	P23895
GO	GO:0016021 GO:0005887 GO:0016020 GO:0015199 GO:0015297 GO:0015220 GO:0015307 GO:0022857 GO:0006974 GO:0015871 GO:0046618 GO:0031460 GO:0042493 GO:0006970 GO:0055085 GO:0006810 GO:0006805
InterPro	IPR000390
Pfam	PF00893

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0022857	transmembrane transporter activity	PMID:22178925^[1]	ECO:0000314			F	Figure 1 talks about EmrE'S changes in conformation to allow substrates in.	complete CACAO 5182
enables	GO:0022857	transmembrane transporter activity	PMID:22178925^[1]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0055085	transmembrane transport	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN001254638 UniProtKB:P9WGF1	P	Seeded From UniProt	complete
involved_in	GO:0031460	glycine betaine transport	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254648	P	Seeded From UniProt	complete
enables	GO:0022857	transmembrane transporter activity	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254638 UniProtKB:P9WGF1	F	Seeded From UniProt	complete
involved_in	GO:0015871	choline transport	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254648	P	Seeded From UniProt	complete
enables	GO:0015297	antiporter activity	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254648	F	Seeded From UniProt	complete
enables	GO:0015220	choline transmembrane transporter activity	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254648	F	Seeded From UniProt	complete
enables	GO:0015199	amino-acid betaine transmembrane transporter activity	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 PANTHER:PTN001254648	F	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10629 EcoGene:EG13926 PANTHER:PTN001254638	C	Seeded From UniProt	complete
enables	GO:0042802	identical protein binding	PMID:20541505^[3]	ECO:0000353	physical interaction evidence used in manual assertion	UniProtKB:P23895	F	Seeded From UniProt	complete
enables	GO:0042802	identical protein binding	PMID:18024586^[4]	ECO:0000353	physical interaction evidence used in manual assertion	UniProtKB:P23895	F	Seeded From UniProt	complete
involved_in	GO:0042493	response to drug	PMID:7896833^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	PMID:9688273^[6]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0016020	membrane	PMID:12356478^[7]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0015297	antiporter activity	PMID:7896833^[5]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0006974	cellular response to DNA damage stimulus	PMID:11967071^[8]	ECO:0000270	expression pattern evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006805	xenobiotic metabolic process	PMID:1320256^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0031460	glycine betaine transport	PMID:22942246^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0015871	choline transport	PMID:22942246^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0015307	obsolete drug:proton antiporter activity	PMID:7896833^[5]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0015307	obsolete drug:proton antiporter activity	PMID:15371426^[11]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0015220	choline transmembrane transporter activity	PMID:22942246^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0015199	amino-acid betaine transmembrane transporter activity	PMID:22942246^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0006970	response to osmotic stress	PMID:22942246^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:9688273^[6]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:7896833^[5]	ECO:0000245	automatically integrated combinatorial evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:15919996^[12]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0046618	drug export	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0015307	P	Seeded From UniProt	complete
involved_in	GO:0046618	drug export	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0015307	P	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000390	C	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C	Seeded From UniProt	complete
enables	GO:0015297	antiporter activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0050	F	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000037 GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1003 UniProtKB-KW:KW-0997 UniProtKB-SubCell:SL-0037	C	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 Morrison, EA et al. (2012) Antiparallel EmrE exports drugs by exchanging between asymmetric structures. Nature 481 45-50 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ Korkhov, VM et al. (2010) Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals. Structure 18 677-87 PubMed GONUTS page
↑ Chen, YJ et al. (2007) X-ray structure of EmrE supports dual topology model. Proc. Natl. Acad. Sci. U.S.A. 104 18999-9004 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 Yerushalmi, H et al. (1995) EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents. J. Biol. Chem. 270 6856-63 PubMed GONUTS page
↑ ^6.0 ^6.1 Schwaiger, M et al. (1998) NMR investigation of the multidrug transporter EmrE, an integral membrane protein. Eur. J. Biochem. 254 610-9 PubMed GONUTS page
↑ Winstone, TL et al. (2002) Optimization of expression and the purification by organic extraction of the integral membrane protein EmrE. Protein Expr. Purif. 26 111-21 PubMed GONUTS page
↑ Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed GONUTS page
↑ Morimyo, M et al. (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20 3159-65 PubMed GONUTS page
↑ ^10.0 ^10.1 ^10.2 ^10.3 ^10.4 Bay, DC & Turner, RJ (2012) Small multidrug resistance protein EmrE reduces host pH and osmotic tolerance to metabolic quaternary cation osmoprotectants. J. Bacteriol. 194 5941-8 PubMed GONUTS page
↑ Rotem, D & Schuldiner, S (2004) EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry. J. Biol. Chem. 279 48787-93 PubMed GONUTS page
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page

[PMID:22178925-1] 1.0 ^1.1 Morrison, EA et al. (2012) Antiparallel EmrE exports drugs by exchanging between asymmetric structures. Nature 481 45-50 PubMed GONUTS page

[PMID:21873635-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:20541505-3] Korkhov, VM et al. (2010) Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals. Structure 18 677-87 PubMed GONUTS page

[PMID:18024586-4] Chen, YJ et al. (2007) X-ray structure of EmrE supports dual topology model. Proc. Natl. Acad. Sci. U.S.A. 104 18999-9004 PubMed GONUTS page

[PMID:7896833-5] 5.0 ^5.1 ^5.2 ^5.3 Yerushalmi, H et al. (1995) EmrE, an Escherichia coli 12-kDa multidrug transporter, exchanges toxic cations and H+ and is soluble in organic solvents. J. Biol. Chem. 270 6856-63 PubMed GONUTS page

[PMID:9688273-6] 6.0 ^6.1 Schwaiger, M et al. (1998) NMR investigation of the multidrug transporter EmrE, an integral membrane protein. Eur. J. Biochem. 254 610-9 PubMed GONUTS page

[PMID:12356478-7] Winstone, TL et al. (2002) Optimization of expression and the purification by organic extraction of the integral membrane protein EmrE. Protein Expr. Purif. 26 111-21 PubMed GONUTS page

[PMID:11967071-8] Khil, PP & Camerini-Otero, RD (2002) Over 1000 genes are involved in the DNA damage response of Escherichia coli. Mol. Microbiol. 44 89-105 PubMed GONUTS page

[PMID:1320256-9] Morimyo, M et al. (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20 3159-65 PubMed GONUTS page

[PMID:22942246-10] 10.0 ^10.1 ^10.2 ^10.3 ^10.4 Bay, DC & Turner, RJ (2012) Small multidrug resistance protein EmrE reduces host pH and osmotic tolerance to metabolic quaternary cation osmoprotectants. J. Bacteriol. 194 5941-8 PubMed GONUTS page

[PMID:15371426-11] Rotem, D & Schuldiner, S (2004) EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry. J. Biol. Chem. 279 48787-93 PubMed GONUTS page

[PMID:15919996-12] Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page

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ECOLI:EMRE

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