GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:1320256
| Citation |
Morimyo, M, Hongo, E, Hama-Inaba, H and Machida, I (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20:3159-65 |
|---|---|
| Abstract |
A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (12.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein. |
| Links | |
| Keywords |
Amino Acid Sequence; Antiporters; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA Transposable Elements/genetics; Drug Resistance, Microbial/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Membrane Proteins/chemistry; Membrane Proteins/genetics; Molecular Sequence Data; Paraquat/pharmacology; Plasmids/genetics; Restriction Mapping |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
acts_upstream_of_or_within |
GO:0042908: xenobiotic transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042910: xenobiotic transmembrane transporter activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0006805: xenobiotic metabolic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006805: xenobiotic metabolic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
