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PMID:1320256

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Citation

Morimyo, M, Hongo, E, Hama-Inaba, H and Machida, I (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity. Nucleic Acids Res. 20:3159-65

Abstract

A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (12.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein.

Links

PubMed PMC312453

Keywords

Amino Acid Sequence; Antiporters; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA Transposable Elements/genetics; Drug Resistance, Microbial/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Membrane Proteins/chemistry; Membrane Proteins/genetics; Molecular Sequence Data; Paraquat/pharmacology; Plasmids/genetics; Restriction Mapping

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:EMRE

acts_upstream_of_or_within

GO:0042908: xenobiotic transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:EMRE

enables

GO:0042910: xenobiotic transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:EMRE

acts_upstream_of_or_within

GO:0006805: xenobiotic metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:EMRE

involved_in

GO:0006805: xenobiotic metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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