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DROME:MLE
Contents
Species (Taxon ID) | Drosophila melanogaster (Fruit fly). (7227) | |
Gene Name(s) | mle (synonyms: nap) | |
Protein Name(s) | Dosage compensation regulator
ATP-dependent RNA helicase mle Protein male-less Protein maleless Protein no action potential | |
External Links | ||
UniProt | P24785 | |
EMBL | M74121 AE013599 AE013599 BT003785 BT010267 | |
PIR | B40025 | |
RefSeq | NP_476641.1 NP_724440.1 | |
UniGene | Dm.2901 | |
ProteinModelPortal | P24785 | |
SMR | P24785 | |
BioGrid | 61429 | |
IntAct | P24785 | |
MINT | MINT-768581 | |
PaxDb | P24785 | |
PRIDE | P24785 | |
EnsemblMetazoa | FBtr0086031 | |
GeneID | 35523 | |
KEGG | dme:Dmel_CG11680 | |
CTD | 35523 | |
FlyBase | FBgn0002774 | |
eggNOG | COG1643 | |
GeneTree | ENSGT00760000119189 | |
InParanoid | P24785 | |
KO | K13184 | |
OMA | VDDWIRL | |
OrthoDB | EOG76471V | |
PhylomeDB | P24785 | |
Reactome | REACT_181701 REACT_181718 REACT_181720 REACT_227819 | |
GenomeRNAi | 35523 | |
NextBio | 793836 | |
PRO | PR:P24785 | |
Proteomes | UP000000803 | |
Bgee | P24785 | |
ExpressionAtlas | P24785 | |
GO | GO:0000785 GO:0005694 GO:0072487 GO:0005700 GO:0000805 GO:0016456 GO:0005524 GO:0008026 GO:0003682 GO:0003725 GO:0004386 GO:0003723 GO:0003724 GO:0006200 GO:0048675 GO:0008340 GO:0007549 GO:0009047 GO:0016457 GO:0045433 GO:0008152 GO:0031453 GO:0045944 | |
Gene3D | 3.30.160.20 3.40.50.300 | |
InterPro | IPR011545 IPR002464 IPR014720 IPR011709 IPR007502 IPR014001 IPR001650 IPR027417 | |
Pfam | PF00270 PF00035 PF04408 PF00271 PF07717 | |
SMART | SM00487 SM00358 SM00847 SM00490 | |
SUPFAM | SSF52540 | |
PROSITE | PS00690 PS50137 PS51192 PS51194 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
part_of |
GO:1990904 |
ribonucleoprotein complex |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0004003 |
ATP-dependent DNA helicase activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003725 |
double-stranded RNA binding |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003690 |
double-stranded DNA binding |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:2000373 |
positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0032508 |
DNA duplex unwinding |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:2000765 |
regulation of cytoplasmic translation |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0050684 |
regulation of mRNA processing |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0001069 |
regulatory region RNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:1990904 |
ribonucleoprotein complex |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001053532 |
C |
Seeded From UniProt |
complete | ||
involved_in |
GO:0050684 |
regulation of mRNA processing |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001053532 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0002774 |
P |
Seeded From UniProt |
complete | ||
enables |
GO:0043140 |
ATP-dependent 3'-5' DNA helicase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001053532 |
F |
Seeded From UniProt |
complete | ||
enables |
GO:0034459 |
ATP-dependent 3'-5' RNA helicase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001449167 |
F |
Seeded From UniProt |
complete | ||
part_of |
GO:0005730 |
nucleolus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:108177 |
C |
Seeded From UniProt |
complete | ||
part_of |
GO:0005634 |
nucleus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0002774 |
C |
Seeded From UniProt |
complete | ||
enables |
GO:0004003 |
ATP-dependent DNA helicase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN001053532 |
F |
Seeded From UniProt |
complete | ||
enables |
GO:0003723 |
RNA binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0002774 |
F |
Seeded From UniProt |
complete | ||
part_of |
GO:0000228 |
nuclear chromosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0007549 |
dosage compensation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0072487 |
MSL complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0003723 |
RNA binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
FB:FBgn0019660 |
F |
Seeded From UniProt |
complete | ||
involved_in |
GO:0031453 |
positive regulation of heterochromatin assembly |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
FB:FBgn0003598 |
P |
Seeded From UniProt |
complete | ||
part_of |
GO:0000805 |
X chromosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0000805 |
X chromosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0008340 |
determination of adult lifespan |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0048675 |
axon extension |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0003724 |
RNA helicase activity |
ECO:0000303 |
author statement without traceable support used in manual assertion |
F |
Seeded From UniProt |
complete | |||
GO:0034459 |
ATP-dependent 3'-5' RNA helicase activity |
ECO:0000315 |
F |
Figure 1D shows the RNA unwinding (helicase) activity of the MLE protein. Two different double stranded RNA substrates are used: one has U-rich 3’ overhang, and the other one is blunt ended. When ATP is added, MLE is found to unwind effectively the dsRNA with e U-rich 3’ overhang (Top panel). In contrast, the blunt ended dsRNA was not separated, despite retaining some unwinding activity. When a deletion is made on MLE domain dsRBD2 (dsRNA binding domain), MLE loses completely its helicase activity (bottom panel). Figure S1C tested helicase activity of MLE with a dsRNA substrate with a 5’ single stranded extension, instead of the 3’ extension used in 1D. MLE did not unwind this substrate, proving that the directionality of MLE helicase activity is 3’ to 5’. MLE is an ATP dependent dsRNA helicase that has specificity for U-rich regions on its RNA substrate and requires a 3’ overhang for activity. |
complete | |||||
enables |
GO:0004386 |
helicase activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
Missing: with/from | |||
enables |
GO:0003725 |
double-stranded RNA binding |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
Missing: with/from | |||
involved_in |
GO:0009047 |
dosage compensation by hyperactivation of X chromosome |
ECO:0000303 |
author statement without traceable support used in manual assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0016456 |
X chromosome located dosage compensation complex, transcription activating |
ECO:0000303 |
author statement without traceable support used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0045433 |
male courtship behavior, veined wing generated song production |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
FB:FBgn0263111 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0009047 |
dosage compensation by hyperactivation of X chromosome |
ECO:0000303 |
author statement without traceable support used in manual assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0007549 |
dosage compensation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0000785 |
chromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0003682 |
chromatin binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0005694 |
chromosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0005700 |
polytene chromosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0003676 |
nucleic acid binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0004386 |
helicase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0016457 |
dosage compensation complex assembly involved in dosage compensation by hyperactivation of X chromosome |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0016456 |
X chromosome located dosage compensation complex, transcription activating |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0007549 |
dosage compensation |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0008026 |
ATP-dependent helicase activity |
ECO:0000304 |
author statement supported by traceable reference used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0005694 |
chromosome |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0004386 |
helicase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Aktaş, T et al. (2017) DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature 544 115-119 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ Figueiredo, ML et al. (2014) Non-coding roX RNAs prevent the binding of the MSL-complex to heterochromatic regions. PLoS Genet. 10 e1004865 PubMed GONUTS page
- ↑ Militti, C et al. (2014) UNR facilitates the interaction of MLE with the lncRNA roX2 during Drosophila dosage compensation. Nat Commun 5 4762 PubMed GONUTS page
- ↑ 5.0 5.1 Hallacli, E et al. (2012) Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila. Mol. Cell 48 587-600 PubMed GONUTS page
- ↑ Spierer, A et al. (2008) SU(VAR)3-7 links heterochromatin and dosage compensation in Drosophila. PLoS Genet. 4 e1000066 PubMed GONUTS page
- ↑ Smith, ER et al. (2008) Regulation of the transcriptional activity of poised RNA polymerase II by the elongation factor ELL. Proc. Natl. Acad. Sci. U.S.A. 105 8575-9 PubMed GONUTS page
- ↑ 8.0 8.1 8.2 Aratani, S et al. (2008) MLE activates transcription via the minimal transactivation domain in Drosophila. Int. J. Mol. Med. 21 469-76 PubMed GONUTS page
- ↑ Kotlikova, IV et al. (2006) The Drosophila dosage compensation complex binds to polytene chromosomes independently of developmental changes in transcription. Genetics 172 963-74 PubMed GONUTS page
- ↑ Fergestad, T et al. (2006) Neuropathology in Drosophila membrane excitability mutants. Genetics 172 1031-42 PubMed GONUTS page
- ↑ Zhong, Y & Wu, CF (2004) Neuronal activity and adenylyl cyclase in environment-dependent plasticity of axonal outgrowth in Drosophila. J. Neurosci. 24 1439-45 PubMed GONUTS page
- ↑ 12.0 12.1 Wutz, A (2003) RNAs templating chromatin structure for dosage compensation in animals. Bioessays 25 434-42 PubMed GONUTS page
- ↑ Prabu, JR et al. (2015) Structure of the RNA Helicase MLE Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling. Mol. Cell 60 487-99 PubMed GONUTS page
- ↑ 14.0 14.1 Lasko, P (2000) The drosophila melanogaster genome: translation factors and RNA binding proteins. J. Cell Biol. 150 F51-6 PubMed GONUTS page
- ↑ 15.0 15.1 Schütt, C & Nöthiger, R (2000) Structure, function and evolution of sex-determining systems in Dipteran insects. Development 127 667-77 PubMed GONUTS page
- ↑ Peixoto, AA & Hall, JC (1998) Analysis of temperature-sensitive mutants reveals new genes involved in the courtship song of Drosophila. Genetics 148 827-38 PubMed GONUTS page
- ↑ 17.0 17.1 Williamson, A & Lehmann, R (1996) Germ cell development in Drosophila. Annu. Rev. Cell Dev. Biol. 12 365-91 PubMed GONUTS page
- ↑ 18.0 18.1 18.2 18.3 18.4 Bone, JR et al. (1994) Acetylated histone H4 on the male X chromosome is associated with dosage compensation in Drosophila. Genes Dev. 8 96-104 PubMed GONUTS page
- ↑ Kuroda, MI et al. (1991) The maleless protein associates with the X chromosome to regulate dosage compensation in Drosophila. Cell 66 935-47 PubMed GONUTS page
- ↑ Amrein, H (2000) Multiple RNA-protein interactions in Drosophila dosage compensation. Genome Biol. 1 REVIEWS1030 PubMed GONUTS page
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