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DROME:CLASP

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Species (Taxon ID) Drosophila melanogaster (Fruit fly). (7227)
Gene Name(s) chb (synonyms: CLASP, Mast, MESR7, orbit)
Protein Name(s) CLIP-associating protein

Misexpression suppressor of ras 7 Protein Multiple asters Mast Protein Orbit Protein chromosome bows

External Links
UniProt Q9NBD7
EMBL AF250842
AF195498
AB031048
AE014296
AE014296
AE014296
AY069579
RefSeq NP_524651.2
NP_730596.1
NP_730597.1
UniGene Dm.1413
PDB 4G3A
PDBsum 4G3A
ProteinModelPortal Q9NBD7
SMR Q9NBD7
BioGrid 68704
IntAct Q9NBD7
MINT MINT-955479
STRING 7227.FBpp0088469
iPTMnet Q9NBD7
PaxDb Q9NBD7
PRIDE Q9NBD7
EnsemblMetazoa FBtr0089460
FBtr0089461
FBtr0089462
GeneID 43901
KEGG dme:Dmel_CG32435
CTD 43901
FlyBase FBgn0021760
eggNOG KOG2956
ENOG410ZMY0
GeneTree ENSGT00390000001762
InParanoid Q9NBD7
KO K16578
OMA CIVKLYF
OrthoDB EOG091G026T
PhylomeDB Q9NBD7
GenomeRNAi 43901
PRO PR:Q9NBD7
Proteomes UP000000803
Bgee FBgn0021760
Genevisible Q9NBD7
GO GO:0005813
GO:0000775
GO:0032154
GO:0005737
GO:0005881
GO:0045169
GO:0045172
GO:0030426
GO:0005828
GO:0005875
GO:0005815
GO:0035371
GO:0005634
GO:0005827
GO:0005876
GO:0000922
GO:0005525
GO:0008017
GO:0051315
GO:0007411
GO:0007282
GO:0040001
GO:0035099
GO:0007067
GO:0000070
GO:0090307
GO:0000022
GO:0007052
GO:0046580
GO:0016325
GO:0048477
GO:0030723
GO:0031116
GO:0046602
GO:0051225
GO:0007051
GO:0019827
Gene3D 1.25.10.10
InterPro IPR011989
IPR016024
IPR028399
IPR024395
IPR021133
IPR034085
PANTHER PTHR21567:SF49
Pfam PF12348
SMART SM01349
SUPFAM SSF48371
PROSITE PS50077

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000022

mitotic spindle elongation

PMID:12034769[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0000022

mitotic spindle elongation

PMID:16303556[2]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0000070

mitotic sister chromatid segregation

PMID:10899121[3]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0000775

chromosome, centromeric region

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0000922

spindle pole

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005525

GTP binding

PMID:10747094[5]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

GO:0005634

nucleus

PMID:10747094[5]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005634

nucleus

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005737

cytoplasm

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0963

C

Seeded From UniProt

GO:0005737

cytoplasm

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005737

cytoplasm

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005813

centrosome

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005815

microtubule organizing center

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0048

C

Seeded From UniProt

GO:0005815

microtubule organizing center

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005819

spindle

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0251

C

Seeded From UniProt

GO:0005827

polar microtubule

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005828

kinetochore microtubule

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR028399

C

Seeded From UniProt

GO:0005856

cytoskeleton

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0206

C

Seeded From UniProt

GO:0005856

cytoskeleton

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0090

C

Seeded From UniProt

GO:0005874

microtubule

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0493

C

Seeded From UniProt

GO:0005875

microtubule associated complex

PMID:10747094[5]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005876

spindle microtubule

PMID:10747094[5]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005876

spindle microtubule

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005876

spindle microtubule

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005881

cytoplasmic microtubule

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR028399

C

Seeded From UniProt

GO:0007049

cell cycle

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0131

P

Seeded From UniProt

GO:0007051

spindle organization

PMID:10747094[5]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007052

mitotic spindle organization

PMID:10899121[3]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007052

mitotic spindle organization

PMID:12034769[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007052

mitotic spindle organization

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007067

mitotic nuclear division

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0498

P

Seeded From UniProt

GO:0007067

mitotic nuclear division

PMID:10747094[5]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007067

mitotic nuclear division

PMID:12034769[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007275

multicellular organism development

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0217

P

Seeded From UniProt

GO:0007282

cystoblast division

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0007411

axon guidance

PMID:15207236[6]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0008017

microtubule binding

PMID:10747094[5]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0008017

microtubule binding

PMID:10899121[3]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0016325

oocyte microtubule cytoskeleton organization

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0019827

stem cell population maintenance

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0030154

cell differentiation

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0221

P

Seeded From UniProt

GO:0030426

growth cone

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0288

C

Seeded From UniProt

GO:0030426

growth cone

PMID:25694447[7]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0030723

ovarian fusome organization

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0031116

positive regulation of microtubule polymerization

PMID:23727231[8]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0032154

cleavage furrow

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0467

C

Seeded From UniProt

GO:0035099

hemocyte migration

PMID:20457764[9]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0035371

microtubule plus-end

PMID:23727231[8]

IMP: Inferred from Mutant Phenotype

-

C

Seeded From UniProt

GO:0035371

microtubule plus-end

PMID:25694447[7]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0040001

establishment of mitotic spindle localization

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0042995

cell projection

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0966

C

Seeded From UniProt

GO:0043515

kinetochore binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR028399

F

Seeded From UniProt

GO:0045169

fusome

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0045172

germline ring canal

PMID:12538517[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0046580

negative regulation of Ras protein signal transduction

PMID:11063696[10]

IGI: Inferred from Genetic Interaction

FB:FBgn0003205

P

Seeded From UniProt

GO:0046580

negative regulation of Ras protein signal transduction

PMID:11063696[10]

IGI: Inferred from Genetic Interaction

FB:FBgn0015402

P

Seeded From UniProt

GO:0046602

regulation of mitotic centrosome separation

PMID:12034769[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0048477

oogenesis

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0896

P

Seeded From UniProt

GO:0048477

oogenesis

PMID:12538517[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0051010

microtubule plus-end binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR028399

F

Seeded From UniProt

GO:0051225

spindle assembly

PMID:15240569[11]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0051301

cell division

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0132

P

Seeded From UniProt

GO:0051315

attachment of mitotic spindle microtubules to kinetochore

PMID:12034769[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0090307

mitotic spindle assembly

PMID:23727231[8]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

colocalizes_with

GO:0000776

kinetochore

PMID:17412918[12]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

colocalizes_with

GO:0005819

spindle

PMID:17412918[12]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0045170

spectrosome

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila (fruit fly) Protein: ORBIT (majorly known as CLASP; mentioned as both in paper) Figure 6. A) Orbit is localized on the spectrosome.

complete

GO:0000776

kinetochore

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila (fruit fly) Protein: Orbit (majorly known as Clasp, mentioned as both in paper.) Figure 6. I) Orbit is localized on kinetochores.

complete

GO:0045169

fusome

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein

Figure 2A&B) (A) An early spermatocyte cyst incubated without colchicine. Note the cytoplasmic microtubule structures and distinct Orbit localization on growing fusomes. (B) An early spermatocyte cyst treated with colchicine; a branched fusome structure is intact. we concluded that Orbit localization on the fusome is independent of microtubules and that microtubules are not essential for the maintenance of the fusome structure.

complete

GO:0005737

cytoplasm

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein Figure 1B) Orbit was also concentrated in the cytoplasm of hub cells,

complete

GO:0072686

mitotic spindle

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein

Figure 1A) The protein was further detected on mitotic spindles and kinetochores

complete

GO:0031965

nuclear membrane

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2A: Orbit was localized around the nuclear membranes during the prophase

complete

GO:0070732

spindle envelope

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2B: Orbit was localized around the nuclear membranes during the prophase and associated with spindle envelopes and kinetochores from the prometaphase to the metaphase

complete

GO:0070938

contractile ring

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2D: By the telophase, Orbit had accumulated on the prospective cleavage furrow region and contractile rings; the protein remained on the contractile rings during cytokinesis .

complete

GO:0005876

spindle microtubule

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila Melanogaster Protein: CLIP-associating protein Fig. 2C, D: We confirmed the complete degradation of spindle microtubules and astral microtubules after colchicine treatment.

complete

GO:0000235

astral microtubule

PMID:23520495[13]

IDA: Inferred from Direct Assay

C

Organism: Drosophila Melanogaster Protein: CLIP-associating protein Fig. 2C, D: We confirmed the complete degradation of spindle microtubules and astral microtubules after colchicine treatment.

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 Maiato, H et al. (2002) MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. J. Cell Biol. 157 749-60 PubMed GONUTS page
  2. Goshima, G et al. (2005) Length control of the metaphase spindle. Curr. Biol. 15 1979-88 PubMed GONUTS page
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 Lemos, CL et al. (2000) Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. EMBO J. 19 3668-82 PubMed GONUTS page
  4. 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 Máthé, E et al. (2003) Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis. Development 130 901-15 PubMed GONUTS page
  5. 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Inoue, YH et al. (2000) Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster. J. Cell Biol. 149 153-66 PubMed GONUTS page
  6. Lee, H et al. (2004) The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. Neuron 42 913-26 PubMed GONUTS page
  7. 7.0 7.1 Beaven, R et al. (2015) Drosophila CLIP-190 and mammalian CLIP-170 display reduced microtubule plus end association in the nervous system. Mol. Biol. Cell 26 1491-508 PubMed GONUTS page
  8. 8.0 8.1 8.2 Leano, JB et al. (2013) A cryptic TOG domain with a distinct architecture underlies CLASP-dependent bipolar spindle formation. Structure 21 939-50 PubMed GONUTS page
  9. Stramer, B et al. (2010) Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo. J. Cell Biol. 189 681-9 PubMed GONUTS page
  10. 10.0 10.1 Huang, AM & Rubin, GM (2000) A misexpression screen identifies genes that can modulate RAS1 pathway signaling in Drosophila melanogaster. Genetics 156 1219-30 PubMed GONUTS page
  11. Inoue, YH et al. (2004) Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J. Cell Biol. 166 49-60 PubMed GONUTS page
  12. 12.0 12.1 Goshima, G et al. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316 417-21 PubMed GONUTS page
  13. 13.0 13.1 13.2 13.3 13.4 13.5 13.6 13.7 13.8 13.9 Miyauchi, C et al. (2013) Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. PLoS ONE 8 e58220 PubMed GONUTS page