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DROME:CLASP
Contents
| Species (Taxon ID) | Drosophila melanogaster (Fruit fly). (7227) | |
| Gene Name(s) | chb (synonyms: CLASP, Mast, MESR7, orbit) | |
| Protein Name(s) | CLIP-associating protein
Misexpression suppressor of ras 7 Protein Multiple asters Mast Protein Orbit Protein chromosome bows | |
| External Links | ||
| UniProt | Q9NBD7 | |
| EMBL | AF250842 AF195498 AB031048 AE014296 AE014296 AE014296 AY069579 | |
| RefSeq | NP_524651.2 NP_730596.1 NP_730597.1 | |
| UniGene | Dm.1413 | |
| PDB | 4G3A | |
| PDBsum | 4G3A | |
| ProteinModelPortal | Q9NBD7 | |
| SMR | Q9NBD7 | |
| BioGrid | 68704 | |
| IntAct | Q9NBD7 | |
| MINT | MINT-955479 | |
| STRING | 7227.FBpp0088469 | |
| iPTMnet | Q9NBD7 | |
| PaxDb | Q9NBD7 | |
| PRIDE | Q9NBD7 | |
| EnsemblMetazoa | FBtr0089460 FBtr0089461 FBtr0089462 | |
| GeneID | 43901 | |
| KEGG | dme:Dmel_CG32435 | |
| CTD | 43901 | |
| FlyBase | FBgn0021760 | |
| eggNOG | KOG2956 ENOG410ZMY0 | |
| GeneTree | ENSGT00390000001762 | |
| InParanoid | Q9NBD7 | |
| KO | K16578 | |
| OMA | CIVKLYF | |
| OrthoDB | EOG091G026T | |
| PhylomeDB | Q9NBD7 | |
| GenomeRNAi | 43901 | |
| PRO | PR:Q9NBD7 | |
| Proteomes | UP000000803 | |
| Bgee | FBgn0021760 | |
| Genevisible | Q9NBD7 | |
| GO | GO:0005813 GO:0000775 GO:0032154 GO:0005737 GO:0005881 GO:0045169 GO:0045172 GO:0030426 GO:0005828 GO:0005875 GO:0005815 GO:0035371 GO:0005634 GO:0005827 GO:0005876 GO:0000922 GO:0005525 GO:0008017 GO:0051315 GO:0007411 GO:0007282 GO:0040001 GO:0035099 GO:0007067 GO:0000070 GO:0090307 GO:0000022 GO:0007052 GO:0046580 GO:0016325 GO:0048477 GO:0030723 GO:0031116 GO:0046602 GO:0051225 GO:0007051 GO:0019827 | |
| Gene3D | 1.25.10.10 | |
| InterPro | IPR011989 IPR016024 IPR028399 IPR024395 IPR021133 IPR034085 | |
| PANTHER | PTHR21567:SF49 | |
| Pfam | PF12348 | |
| SMART | SM01349 | |
| SUPFAM | SSF48371 | |
| PROSITE | PS50077 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0045170 |
spectrosome |
ECO:0000314 |
C |
Organism: Drosophila (fruit fly) Protein: ORBIT (majorly known as CLASP; mentioned as both in paper) Figure 6. A) Orbit is localized on the spectrosome. |
complete | |||||
| GO:0000776 |
kinetochore |
ECO:0000314 |
C |
Organism: Drosophila (fruit fly) Protein: Orbit (majorly known as Clasp, mentioned as both in paper.) Figure 6. I) Orbit is localized on kinetochores. |
complete | |||||
| GO:0045169 |
fusome |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Figure 2A&B) (A) An early spermatocyte cyst incubated without colchicine. Note the cytoplasmic microtubule structures and distinct Orbit localization on growing fusomes. (B) An early spermatocyte cyst treated with colchicine; a branched fusome structure is intact. we concluded that Orbit localization on the fusome is independent of microtubules and that microtubules are not essential for the maintenance of the fusome structure. |
complete | |||||
| GO:0005737 |
cytoplasm |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Figure 1B) Orbit was also concentrated in the cytoplasm of hub cells, |
complete | |||||
| GO:0072686 |
mitotic spindle |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Figure 1A) The protein was further detected on mitotic spindles and kinetochores |
complete | |||||
| GO:0031965 |
nuclear membrane |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2A: Orbit was localized around the nuclear membranes during the prophase |
complete | |||||
| GO:0070732 |
spindle envelope |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2B: Orbit was localized around the nuclear membranes during the prophase and associated with spindle envelopes and kinetochores from the prometaphase to the metaphase |
complete | |||||
| GO:0070938 |
contractile ring |
ECO:0000314 |
C |
Organism: Drosophila melanogaster Protein: CLIP-associating protein Fig. S2D: By the telophase, Orbit had accumulated on the prospective cleavage furrow region and contractile rings; the protein remained on the contractile rings during cytokinesis . |
complete | |||||
|
involved_in |
GO:0031116 |
positive regulation of microtubule polymerization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0005876 |
spindle microtubule |
ECO:0000314 |
C |
Organism: Drosophila Melanogaster Protein: CLIP-associating protein Fig. 2C, D: We confirmed the complete degradation of spindle microtubules and astral microtubules after colchicine treatment. |
complete | |||||
|
involved_in |
GO:0090307 |
mitotic spindle assembly |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0000235 |
astral microtubule |
ECO:0000314 |
C |
Organism: Drosophila Melanogaster Protein: CLIP-associating protein Fig. 2C, D: We confirmed the complete degradation of spindle microtubules and astral microtubules after colchicine treatment. |
complete | |||||
|
part_of |
GO:0035371 |
microtubule plus-end |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0045169 |
fusome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030426 |
growth cone |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0035371 |
microtubule plus-end |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0035099 |
hemocyte migration |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005819 |
spindle |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0000776 |
kinetochore |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000022 |
mitotic spindle elongation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007411 |
axon guidance |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051225 |
spindle assembly |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0016325 |
oocyte microtubule cytoskeleton organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0030723 |
ovarian fusome organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0045172 |
germline ring canal |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007282 |
cystoblast division |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005876 |
spindle microtubule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019827 |
stem cell population maintenance |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007052 |
mitotic spindle organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0040001 |
establishment of mitotic spindle localization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0048477 |
oogenesis |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0000922 |
spindle pole |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000022 |
mitotic spindle elongation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000278 |
mitotic cell cycle |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046602 |
regulation of mitotic centrosome separation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007052 |
mitotic spindle organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051315 |
attachment of mitotic spindle microtubules to kinetochore |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000070 |
mitotic sister chromatid segregation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005815 |
microtubule organizing center |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005813 |
centrosome |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005876 |
spindle microtubule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005827 |
polar microtubule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0000775 |
chromosome, centromeric region |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008017 |
microtubule binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007052 |
mitotic spindle organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000278 |
mitotic cell cycle |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005875 |
microtubule associated complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008017 |
microtubule binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007051 |
spindle organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005525 |
GTP binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005876 |
spindle microtubule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005828 |
kinetochore microtubule |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005881 |
cytoplasmic microtubule |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0043515 |
kinetochore binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051010 |
microtubule plus-end binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0042995 |
cell projection |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0048477 |
oogenesis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007049 |
cell cycle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005856 |
cytoskeleton |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005874 |
microtubule |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051301 |
cell division |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0030154 |
cell differentiation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007275 |
multicellular organism development |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030426 |
growth cone |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0032154 |
cleavage furrow |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005819 |
spindle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005815 |
microtubule organizing center |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Miyauchi, C et al. (2013) Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. PLoS ONE 8 e58220 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Leano, JB et al. (2013) A cryptic TOG domain with a distinct architecture underlies CLASP-dependent bipolar spindle formation. Structure 21 939-50 PubMed GONUTS page
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 Máthé, E et al. (2003) Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis. Development 130 901-15 PubMed GONUTS page
- ↑ 4.0 4.1 Beaven, R et al. (2015) Drosophila CLIP-190 and mammalian CLIP-170 display reduced microtubule plus end association in the nervous system. Mol. Biol. Cell 26 1491-508 PubMed GONUTS page
- ↑ Stramer, B et al. (2010) Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo. J. Cell Biol. 189 681-9 PubMed GONUTS page
- ↑ 6.0 6.1 Goshima, G et al. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316 417-21 PubMed GONUTS page
- ↑ Goshima, G et al. (2005) Length control of the metaphase spindle. Curr. Biol. 15 1979-88 PubMed GONUTS page
- ↑ Lee, H et al. (2004) The microtubule plus end tracking protein Orbit/MAST/CLASP acts downstream of the tyrosine kinase Abl in mediating axon guidance. Neuron 42 913-26 PubMed GONUTS page
- ↑ Inoue, YH et al. (2004) Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression. J. Cell Biol. 166 49-60 PubMed GONUTS page
- ↑ 10.0 10.1 10.2 10.3 10.4 Maiato, H et al. (2002) MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. J. Cell Biol. 157 749-60 PubMed GONUTS page
- ↑ 11.0 11.1 11.2 11.3 11.4 11.5 11.6 11.7 11.8 Lemos, CL et al. (2000) Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. EMBO J. 19 3668-82 PubMed GONUTS page
- ↑ 12.0 12.1 12.2 12.3 12.4 12.5 12.6 Inoue, YH et al. (2000) Orbit, a novel microtubule-associated protein essential for mitosis in Drosophila melanogaster. J. Cell Biol. 149 153-66 PubMed GONUTS page
a
c
- GO:0007049 ! cell cycle
- GO:0030154 ! cell differentiation
- GO:0051301 ! cell division
- GO:0042995 ! cell projection
- GO:0005813 ! centrosome
- GO:0000775 ! chromosome, centromeric region
- GO:0032154 ! cleavage furrow
- GO:0070938 ! contractile ring
- GO:0007282 ! cystoblast division
- GO:0005737 ! cytoplasm
- GO:0005881 ! cytoplasmic microtubule
- GO:0005856 ! cytoskeleton
e
i
m
- Metazoa
- GO:0005874 ! microtubule
- GO:0051010 ! microtubule plus-end binding
- GO:0035371 ! microtubule plus-end
- GO:0005875 ! microtubule associated complex
- GO:0008017 ! microtubule binding
- GO:0005815 ! microtubule organizing center
- GO:0000278 ! mitotic cell cycle
- GO:0000070 ! mitotic sister chromatid segregation
- GO:0090307 ! mitotic spindle assembly
- GO:0072686 ! mitotic spindle
- GO:0007052 ! mitotic spindle organization
- GO:0000022 ! mitotic spindle elongation
- GO:0007275 ! multicellular organism development
- Muscomorpha
o
p
