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PMID:10899121

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Citation

Lemos, CL, Sampaio, P, Maiato, H, Costa, M, Omel'yanchuk, LV, Liberal, V and Sunkel, CE (2000) Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization. EMBO J. 19:3668-82

Abstract

Through mutational analysis in Drosopjila we have identified the gene multiple asters (mast), which encodes a new 165 kDa protein. mast mutant neuroblasts are highly polyploid and show severe mitotic abnormalities including the formation of mono- and multi-polar spindles organized by an irregular number of microtubule-organizing centres of abnormal size and shape. The mast gene product is evolutionarily conserved since homologues were identified from yeast to man, revealing a novel protein family. Antibodies against Mast and analysis of tissue culture cells expressing an enhanced green fluorescent protein-Mast fusion protein show that during mitosis, this protein localizes to centrosomes, the mitotic spindle, centromeres and spindle midzone. Microtubule-binding assays indicate that Mast is a microtubule-associated protein displaying strong affinity for polymerized microtubules. The defects observed in the mutant alleles and the intracellular localization of the protein suggest that Mast plays an essential role in centrosome separation and organization of the bipolar mitotic spindle.

Links

PubMed PMC313969 Online version:10.1093/emboj/19.14.3668

Keywords

Amino Acid Sequence; Animals; Brain/cytology; Brain/embryology; Brain/metabolism; Cells, Cultured; Centrosome/chemistry; Centrosome/drug effects; Centrosome/metabolism; Cloning, Molecular; Colchicine/pharmacology; Conserved Sequence; Drosophila Proteins; Drosophila melanogaster/drug effects; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Evolution, Molecular; Fluorescent Antibody Technique; Green Fluorescent Proteins; Humans; Insect Proteins/chemistry; Insect Proteins/genetics; Insect Proteins/metabolism; Luminescent Proteins; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Mitosis/drug effects; Mitosis/physiology; Mitotic Spindle Apparatus/chemistry; Mitotic Spindle Apparatus/drug effects; Mitotic Spindle Apparatus/metabolism; Molecular Sequence Data; Mutation; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Phenotype; Phylogeny; Protein Binding; Recombinant Fusion Proteins; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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