Category:Team Green-B

Figure 2. Using a GFP variant of SpmX (SpmX-eYFP), Perez et al. assayed for subcellular localization of PopZ and SpmX proteins. SpmX-eYFP was found to be distributed throughout the cell but when coexpressed with mCherry-PopZ, the two localized at the cellular poles.

Figure 2. Using a GFP variant of PopZ (mCherry-PopZ), Perez et al. assayed for subcellular localization of PopZ and SpmX proteins. mCherry-PopZ was found to localize to one cell pole.

Figures 2 and 3. Takeuchi et al. found that ΦSA012TM103 and ΦSA012TM20 mutant variants of ΦSA012 could not infect host RN4220. ΦSA012TM103 only produced turbid plaques when plated with RN4220 and ΦSA012TM20 could not produce plaques at all. The reason being that RN4220 has the tarM gene which produces α-GlcNAc modification of WTAs. With this modification ΦSA012 mutants cannot infect RN4220. RN4220 with tarM deleted became susceptible to both ΦSA012 mutants as shown in spot tests and adsorption assays.

Figure 6. Takeuchi et al. found that 2 mutations, M103 and M20, in orf103 of phage ΦSA012 enhanced adsorption allowing infection of the normal ΦSA012-resistant organisms that they would otherwise not be able to infect. This was confirmed by adsorption assay and spot testing.

Figure 5. Takeuchi et al. used gold-conjugated anti-orf103 antibodies known to specifically bind to the orf103 gene product. Gold-bound Ab allowed for visualization of the location via immunoelectron microscopy. They found that the gene product localized in the tail fiber at the bottom of the baseplate.