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9CAUD:W0TUZ2
Contents
| Species (Taxon ID) | Staphylococcus phage phiSA12. (1450142) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | Virion component, putative tail fiber (ECO:0000313 with EMBL:BAO47150.1) | |
| External Links | ||
| UniProt | W0TUZ2 | |
| EMBL | AB903967 | |
| RefSeq | YP_009006774.1 | |
| GeneID | 18500198 | |
| KEGG | vg:18500198 | |
| Proteomes | UP000019126 | |
| GO | GO:0016798 | |
| Gene3D | 2.60.120.260 | |
| InterPro | IPR003305 IPR008979 | |
| Pfam | PF02018 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0098670 |
entry receptor-mediated virion attachment to host cell |
ECO:0000315 |
P |
Figure 6. Takeuchi et al. found that 2 mutations, M103 and M20, in orf103 of phage ΦSA012 enhanced adsorption allowing infection of the normal ΦSA012-resistant organisms that they would otherwise not be able to infect. This was confirmed by adsorption assay and spot testing. |
complete | |||||
| GO:0098024 |
virus tail, fiber |
ECO:0000314 |
C |
Figure 5. Takeuchi et al. used gold-conjugated anti-orf103 antibodies known to specifically bind to the orf103 gene product. Gold-bound Ab allowed for visualization of the location via immunoelectron microscopy. They found that the gene product localized in the tail fiber at the bottom of the baseplate. |
complete | |||||
| GO:0098001 |
receptor-mediated bacteriophage reversible attachment to host cell |
ECO:0000315 |
P |
Figures 2 and 3. Takeuchi et al. found that ΦSA012TM103 and ΦSA012TM20 mutant variants of ΦSA012 could not infect host RN4220. ΦSA012TM103 only produced turbid plaques when plated with RN4220 and ΦSA012TM20 could not produce plaques at all. The reason being that RN4220 has the tarM gene which produces α-GlcNAc modification of WTAs. With this modification ΦSA012 mutants cannot infect RN4220. RN4220 with tarM deleted became susceptible to both ΦSA012 mutants as shown in spot tests and adsorption assays. |
complete | |||||
|
enables |
GO:0016798 |
hydrolase activity, acting on glycosyl bonds |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 Takeuchi, I et al. (2016) The Presence of Two Receptor-Binding Proteins Contributes to the Wide Host Range of Staphylococcal Twort-Like Phages. Appl. Environ. Microbiol. 82 5763-74 PubMed GONUTS page
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