Category:Team Contagious Intelligence

Figure 5 shows the adhesion of C. difficile to Vero cells in the absence and presence of anti-GroEL; adhesion decreases when anti-GroEL is present indicating that GroEL is involved in the attachment of C. difficile to cells.

Table 2.

Fig 4.

Figure 5 shows that a clpP mutant strain has a reduced ability to degrade puromycyl-containing polypeptides which are comparable to misfolded proteins. This indicates that ClpP degrades misfolded proteins.

As seen in figure 5, purified netB protein incubated with chicken or duck red blood cells caused an increase in hemoglobin, implying that hemolysis occurred.

Figure 1B shows adherence of Vero cells to purified GST protein (negative control), FliC, FliD, and the crude flagella. Significant adherence is seen in the purified FliD; a weaker adherence is seen in the crude flagella (both FliC and FliD). These results indicate that FliD has a role in cell adhesion during C. difficile infection.

As seen in figure 4, the SpoIVA mutant exhibits an immature endospore phenotype in contrast with the sporulating wild type.