PMID:16861796

Citation	Miyoshi, Y, Okada, S, Uchimura, T and Satoh, E (2006) A mucus adhesion promoting protein, MapA, mediates the adhesion of Lactobacillus reuteri to Caco-2 human intestinal epithelial cells. Biosci. Biotechnol. Biochem. 70:1622-8
Abstract	Lactobacillus reuteri is one of the dominant lactobacilli found in the gastrointestinal tract of various animals. A surface protein of L. reuteri 104R, mucus adhesion promoting protein (MapA), is considered to be an adhesion factor of this strain. We investigated the relation between MapA and adhesion of L. reuteri to human intestinal (Caco-2) cells. Quantitative analysis of the adhesion of L. reuteri strains to Caco-2 cells showed that various L. reuteri strains bind not only to mucus but also to intestinal epithelial cells. In addition, purified MapA bound to Caco-2 cells, and this binding inhibited the adhesion of L. reuteri in a concentration-dependent manner. Based on these observations, the adhesion of L. reuteri appears due to the binding of MapA to receptor-like molecules on Caco-2 cells. Further, far-western analysis indicated the existence of multiple receptor-like molecules in Caco-2 cells.
Links	PubMed
Keywords	Bacterial Adhesion; Bacterial Proteins/physiology; Caco-2 Cells; Epithelial Cells/physiology; Humans; Intestinal Mucosa/cytology; Lactobacillus reuteri/physiology; Membrane Proteins/physiology; Mucus/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LACRE:Q9FCU5	involved_in	GO:0044650: adhesion of symbiont to host cell	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
LACRE:Q9FCU5		GO:0044650: adhesion of symbiont to host cell	ECO:0000314:		P		Fig 4.	complete CACAO 7790

References

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