9CAUD:S5Y0G6

Species (Taxon ID)	Bacillus phage Troll. (1382932)
Gene Name(s)	44 (ECO:0000313 with EMBL:AGT13569.1)
Protein Name(s)	Dihydrofolate reductase (ECO:0000313 with EMBL:AGT13569.1)
External Links
UniProt	S5Y0G6
EMBL	KF208639
RefSeq	YP_008431117.1
GeneID	16575543
GO	GO:0004146 GO:0050661 GO:0006545 GO:0009165
Gene3D	3.40.430.10
InterPro	IPR012259 IPR024072 IPR001796
Pfam	PF00186
PIRSF	PIRSF000194
PRINTS	PR00070
SUPFAM	SSF53597
PROSITE	PS51330

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0004146	dihydrofolate reductase activity	GO_REF:0000100	ECO:0000247		UniProtKB:P0ABQ4	F		Focused blastp result with e-value 4e-31, query coverage 88%, and identity 41% identifies Escherichia coli str. K-12 dihydrofolate reductase as a homolog for this gene product. Dihydrofolate reductase activity of E. coli K-12 DHFR was found by performing a direct dihydrofolate reductase assay. Conserved residues for the gene product in E. coli (e.g. Ala-7, Phe-31, Leu-54, etc.) had identical residue matches in Troll.	complete CACAO 10866
	GO:0004146	dihydrofolate reductase activity	GO_REF:0000100	ECO:0000247		PMID:19950924 UniProtKB:O85255_STREE	F		HHPred showed promising alignment with e value=5E-45, p value=2.6E-50, and prob =100.0. This gene is a putative homologue of the prior one as evidenced by the alignment and shared function. Primary paper supports DHFR activity by mutant phenotpe. "e KM values of NADPH and H2F for the wild type spDHFR and the mutant Sp9 were measured (Table 2B). The KM values for the wild type spDHFR were at least order of magnitude higher than those for the Sp9 mutant that showed full enzyme activity even at a limiting low concentration of 0.63 μM H2F consistent with a much lower KM value for this substrate. "	complete CACAO 10892
enables	GO:0004146	dihydrofolate reductase activity	GO_REF:0000100	ECO:0000247	sequence alignment evidence used in manual assertion	UniProtKB:P0ABQ4	F		Seeded From UniProt	complete
enables	GO:0004146	dihydrofolate reductase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001796 InterPro:IPR012259	F		Seeded From UniProt	complete
involved_in	GO:0006545	glycine biosynthetic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012259	P		Seeded From UniProt	complete
involved_in	GO:0046654	tetrahydrofolate biosynthetic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001796 InterPro:IPR012259	P		Seeded From UniProt	complete
enables	GO:0050661	NADP binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR012259	F		Seeded From UniProt	complete
involved_in	GO:0055114	oxidation-reduction process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001796 InterPro:IPR012259	P		Seeded From UniProt	complete
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References

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