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GO:0004146dihydrofolate reductase activityother:AGT13569.2ISA: Inferred from Sequence Alignment PMID:19950924 UniProtKB:O85255_STREE F
This annotation made on page: 9CAUD:S5Y0G6
By: Mhassan (group TeamGreen) on 2015-04-08 08:17:31 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Scaruso2015-05-12 13:49:32 CDT

This should have been a regular annotation of Streptococcus pneumoniae DHFR from the ref, not a transfer to Troll (9CAUD:S5Y0G6).

Unacceptable
Publication
Qualifier
Go term
Evidence
Notes
Public
Assessment
DanielRenfro2015-05-11 13:52:40 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004146 dihydrofolate reductase activity PMID:19950924 ISA: Inferred from Sequence Alignment PMID:19950924

UniProtKB:O85255_STREE

F HHPred showed promising alignment with e value=5E-45, p value=2.6E-50, and prob =100.0. This gene is a putative homologue of the prior one as evidenced by the alignment and shared function. Primary paper supports DHFR activity by mutant phenotpe. "e KM values of NADPH and H2F for the wild type spDHFR and the mutant Sp9 were measured (Table 2B). The KM values for the wild type spDHFR were at least order of magnitude higher than those for the Sp9 mutant that showed full enzyme activity even at a limiting low concentration of 0.63 μM H2F consistent with a much lower KM value for this substrate. " complete
CACAO 10892
on 9CAUD:S5Y0G6
Flagged
Public
Assessment
Scaruso2015-05-03 16:30:26 CDT

Please add to these notes. Be more specific than one matches another. What (what gene from what organism) matches what by the numbers you gave? Check the reference, the link is not working.

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