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XANAC:Q8PHY8

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Species (Taxon ID) Xanthomonas axonopodis pv. citri (strain 306). (190486)
Gene Name(s) No Information Provided.
Protein Name(s) Glycosyltransferase (ECO:0000313 with EMBL:AAM37955.1)
External Links
UniProt Q8PHY8
EMBL AE008923
RefSeq NP_643419.2
ProteinModelPortal Q8PHY8
STRING 190486.XAC3110
EnsemblBacteria AAM37955
GeneID 1157181
KEGG xac:XAC3110
PATRIC 24058355
HOGENOM HOG000253081
OMA PAYFEDT
OrthoDB EOG6Z99WQ
BioCyc XAXO190486:GH55-3110-MONOMER
Proteomes UP000000576
GO GO:0016740
GO:0009405
GO:0044407
GO:0044011
Gene3D 3.90.550.10
InterPro IPR001173
IPR029044
Pfam PF00535
SUPFAM SSF53448

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009405

pathogenesis

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Xanthomonas axonopodis Glycosyltransferase (gpsX)

The virulence of the gpsX mutant was assessed on the host plant grapefruit using two inoculation methods: pressure infiltration and spray. As seen in figure 4A, upon infiltration at a concentration 105 colony-forming units (cfu)/ml the gpsX mutant induced less canker lesions than the wild-type strain 306 at 14 dpi. When infiltrated at a higher concentration (108 cfu/ml), the gpsX mutant induced significantly less lesions than wild type at 7 dpi, but caused similar disease symptoms as wild type at 14 dpi. Plant inoculation by spray showed that the gpsX mutant was reduced in virulence on grapefruit compared with the wild-type strain 306. After 21 days post inoculation the number of canker lesions on leaves infected with the gpsX mutant was significantly less than that inoculated with wild type strain.

complete
CACAO 4416

GO:0048870

cell motility

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 7, the gpsX mutant was evaluated for the mobile ability on swimming plates. The results showed that a significant reduction in swimming motility was observed in the gpsX mutant 223 G4 (gpsX-), compared with the wildtype strain.

complete
CACAO 4417

GO:0044011

single-species biofilm formation on inanimate substrate

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 6A and 6B, the gpsX mutant was examined on polystyrene and glass surfaces. The gpsX mutant 223 G4 (gpsX-) exhibited a significant reduction in biofilm formation both on polystyrene surface and in glass tubes compared to that of the wild-type, where the level of biofilm formation were approximately 30% and 40% of the wild-type level.

complete
CACAO 4419

GO:0044407

single-species biofilm formation in or on host organism

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 6C, the gpsX mutant showed declined biofilm formation on citrus leaf surfaces as compared to the wild type.

complete
CACAO 4420

GO:0009279

cell outer membrane

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

C

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 3B, a sodium dodecylsulphate-polyacrylamide gel electrophoresis analysis showed that LPS of the gpsX mutant was different from that of the wild-type strain 306. Two bands corresponding to the O-antigen containing LPS were completely lost in the gpsX mutant, compared to wild type strain 306.

complete
CACAO 4585

GO:0008152

metabolic process

GO_REF:0000038

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0808

P

Seeded From UniProt

complete

GO:0009405

pathogenesis

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0016740

transferase activity

GO_REF:0000038

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

GO:0033554

cellular response to stress

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in table 4, after 20 min of exposure to UV radiation, there were greater numbers of surviving cells of the wild-type strain than that of the gpsX mutant.

complete

GO:0044011

single-species biofilm formation on inanimate substrate

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0044407

single-species biofilm formation in or on host organism

PMID:22404966[1]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Li, J & Wang, N (2012) The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri. BMC Microbiol. 12 31 PubMed GONUTS page