PMID:22404966

Citation	Li, J and Wang, N (2012) The gpsX gene encoding a glycosyltransferase is important for polysaccharide production and required for full virulence in Xanthomonas citri subsp. citri. BMC Microbiol. 12:31
Abstract	The Gram-negative bacterium Xanthomonas citri subsp. citri (Xac) causes citrus canker, one of the most destructive diseases of citrus worldwide. In our previous work, a transposon mutant of Xac strain 306 with an insertion in the XAC3110 locus was isolated in a screening that aimed at identifying genes related to biofilm formation. The XAC3110 locus was named as bdp24 for biofilm-defective phenotype and the mutant was observed to be affected in extracellular polysaccharide (EPS) and lipopolysaccharide (LPS) biosynthesis and cell motility. In this study, we further characterized the bdp24 (XAC3110) gene (designated as gpsX) using genetic complementation assays and expanded the knowledge about the function of the gpsX gene in Xac pathogenesis by investigating the roles of gpsX in EPS and LPS production, cell motility, biofilm formation on host leaves, stress tolerance, growth in planta, and host virulence of the citrus canker bacterium.
Links	PubMed PMC3364877 Online version:10.1186/1471-2180-12-31
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Citrus/microbiology; Genetic Complementation Test; Glycosyltransferases/genetics; Glycosyltransferases/metabolism; Molecular Sequence Data; Plant Diseases/microbiology; Polysaccharides, Bacterial/biosynthesis; Sequence Alignment; Virulence; Xanthomonas/enzymology; Xanthomonas/genetics; Xanthomonas/pathogenicity
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
XANAC:Q8PHY8		GO:0009405: pathogenesis	ECO:0000315:		P		Xanthomonas axonopodis Glycosyltransferase (gpsX) The virulence of the gpsX mutant was assessed on the host plant grapefruit using two inoculation methods: pressure infiltration and spray. As seen in figure 4A, upon infiltration at a concentration 105 colony-forming units (cfu)/ml the gpsX mutant induced less canker lesions than the wild-type strain 306 at 14 dpi. When infiltrated at a higher concentration (108 cfu/ml), the gpsX mutant induced significantly less lesions than wild type at 7 dpi, but caused similar disease symptoms as wild type at 14 dpi. Plant inoculation by spray showed that the gpsX mutant was reduced in virulence on grapefruit compared with the wild-type strain 306. After 21 days post inoculation the number of canker lesions on leaves infected with the gpsX mutant was significantly less than that inoculated with wild type strain.	complete CACAO 4416
XANAC:Q8PHY8		GO:0048870: cell motility	ECO:0000315:		P		Xanthomonas axonopodis Glycosyltransferase (gpsX) As seen in figure 7, the gpsX mutant was evaluated for the mobile ability on swimming plates. The results showed that a significant reduction in swimming motility was observed in the gpsX mutant 223 G4 (gpsX-), compared with the wildtype strain.	complete CACAO 4417
XANAC:Q8PHY8		GO:0044011: single-species biofilm formation on inanimate substrate	ECO:0000315:		P		Xanthomonas axonopodis Glycosyltransferase (gpsX) As seen in figure 6A and 6B, the gpsX mutant was examined on polystyrene and glass surfaces. The gpsX mutant 223 G4 (gpsX-) exhibited a significant reduction in biofilm formation both on polystyrene surface and in glass tubes compared to that of the wild-type, where the level of biofilm formation were approximately 30% and 40% of the wild-type level.	complete CACAO 4419
XANAC:Q8PHY8		GO:0044407: single-species biofilm formation in or on host organism	ECO:0000315:		P		Xanthomonas axonopodis Glycosyltransferase (gpsX) As seen in figure 6C, the gpsX mutant showed declined biofilm formation on citrus leaf surfaces as compared to the wild type.	complete CACAO 4420
XANAC:Q8PHY8		GO:0009279: cell outer membrane	ECO:0000315:		C		Xanthomonas axonopodis Glycosyltransferase (gpsX) As seen in figure 3B, a sodium dodecylsulphate-polyacrylamide gel electrophoresis analysis showed that LPS of the gpsX mutant was different from that of the wild-type strain 306. Two bands corresponding to the O-antigen containing LPS were completely lost in the gpsX mutant, compared to wild type strain 306.	complete CACAO 4585
XANAC:Q8PHY8		GO:0033554: cellular response to stress	ECO:0000315:		P		Xanthomonas axonopodis Glycosyltransferase (gpsX) As seen in table 4, after 20 min of exposure to UV radiation, there were greater numbers of surviving cells of the wild-type strain than that of the gpsX mutant.	complete CACAO 12253
XANAC:Q8PHY8	involved_in	GO:0009405: pathogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
XANAC:Q8PHY8	involved_in	GO:0044011: single-species biofilm formation on inanimate substrate	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
XANAC:Q8PHY8	involved_in	GO:0044407: single-species biofilm formation in or on host organism	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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