User:Stankalwala

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	DANRE:E7F8P0	1969-12-31 06:00:00 CST	GO:0006915 apoptotic process (P)	PMID:27050765	ECO:0000314 direct assay evidence used in manual assertion	Seeded from Uniprot	challenge
unacceptable	DANRE:E7F8P0	2016-04-15 10:35:36 CDT	GO:0016567 protein ubiquitination (P)	PMID:27050765		trim69 is ubiquitously expressed in zebrafish embryos’ brain of 24hpf, 48hpf, and 72hpf.	challenge
acceptable	DANRE:A0A060IVL7	2016-04-15 10:51:27 CDT	GO:0022004 midbrain-hindbrain boundary maturation during brain development (P)	PMID:27050765	ECO:0000315 mutant phenotype evidence used in manual assertion	Decrease in Trim69 by Morpholino antisense oligonucleotides induced brain deformation particularly in the mid-hind brain boundary, as shown by figure 2 (a,b).	challenge
updatedbyinstructor	DANRE:A0A060IVL7	2016-04-15 02:18:59 CDT	GO:1904746 negative regulation of apoptotic process involved in development (P)	PMID:27050765	ECO:0000315 mutant phenotype evidence used in manual assertion	Trim69-MO lead to a increased apoptotic cells when compared to Trim69. This will lead into a phenotypic degradation of the developing embryo brain in the zebrafish. shown in figure 2: "2(B) The examined apoptosis of zebrafish embryos by TUNEL assay. trim69-CON(e): control group; trim69-MO(f): trim69 knocking down; trim69-MO:trim69-mRNA(g): co-injection with trim69-MO and human trim69 mRNA; black arrow indicates apoptotic cells; scale bar: 200 μm."	challenge
unacceptable	DANRE:A0A060IVL7	2016-04-15 10:53:35 CDT	GO:1900118 negative regulation of execution phase of apoptosis (P)	PMID:2705076	ECO:0000316 genetic interaction evidence used in manual assertion	TUNEL assay analyses indicated that the human TRIM69 mRNA can also rescue the observed apoptosis induced by zebrafish trim69-MO. ALSO qPCR assay analyses indicated that the human trim69 mRNA can rescue the apoptosis induced by zebrafish trim69-MO.	challenge
unacceptable	DANRE:Q4ZJE9	2016-04-12 12:07:07 CDT	GO:0022004 midbrain-hindbrain boundary maturation during brain development (P)	PMID:27050765	ECO:0000315 mutant phenotype evidence used in manual assertion	Loss of c-Jun rescued the deformed brain induced by trim69 knockdown shown in figure	challenge
unacceptable	DANRE:Q4ZJE9	2016-04-12 01:54:09 CDT	GO:0022004 midbrain-hindbrain boundary maturation during brain development (P)	PMID:27050765	ECO:0000315 mutant phenotype evidence used in manual assertion	Loss of c-Jun rescued the deformed brain induced by trim69 knockdown shown in figure 7	challenge
unacceptable	DANRE:Q4ZJE9	2016-04-12 01:54:09 CDT	GO:0072089 stem cell proliferation (P)	PMID:27050765	ECO:0000314 direct assay evidence used in manual assertion	qPCR showed the expression for stem cell markers can be rescued by co-injection with c-Jun-MO and this match with the in situ hybridization shown in figure 3	challenge

acceptable:1
unacceptable:6
requires_changes:0
flagged:0

Annotations challenged by Stankalwala

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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