GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

User:Sphanor

From GONUTS
Jump to: navigation, search

CACAO Spring 2018

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorMOUSE:ARP212018-04-12 00:17:51 CDTGO:0005737 cytoplasm (C)PMID:29581509ECO:0000314 direct assay evidence used in manual assertion

ARPP21 localizes to the cytosol and overlaps staining with stress granule. ARPP21 used FLAG-tagg expression in HeLa cells and treated with arsenite Figure 2A,B

challenge
updatedbyinstructorHUMAN:LARP12018-04-13 00:04:20 CDTGO:0045948 positive regulation of translational initiation (P)PMID:29244122ECO:0000315 mutant phenotype evidence used in manual assertion

Luciferase reporter mRNA is sensitive to competition with cap analog and translation in mTOR-inhibited extracts is less. The 5'end of mRNA was truncated by encoding hammerhead ribozyme upstream of 5'UTR. Primer extension assays proved ligation efficiency of 90%. Translation of TOP reporter is more repressed in mTOR inhibited extracts. This means no/less production of luciferase.

figure 1 D,E and Sup. figure 2

challenge

acceptable:0
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Sphanor

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Sphanor

What do the icons mean in the status column?

assessment icons.jpg