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User:Shookjoh

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorVIBCH:Q9KKY52014-04-06 14:49:12 CDTGO:0052621 diguanylate cyclase activity (F)PMID:24490592ECO:0000314 direct assay evidence used in manual assertion

Figure 4 clearly demonstrates VCA0965's ability to synthesize c-di-GMP using Liquid Chromatography and Mass Spectrophotometry. Also see Fig 5.

challenge
updatedbyinstructorSERMA:O248232014-04-06 17:00:20 CDTGO:0008800 beta-lactamase activity (F)PMID:9449282ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2

challenge
unacceptableVIBHA:Q9AMP12014-04-06 17:38:04 CDTGO:0006032 chitin catabolic process (P)PMID:18467126ECO:0000315 mutant phenotype evidence used in manual assertion

Figure two shows specific chitin related binding with hydrogen bonds as dashed lines. NAG6 binds in the mutant variants well but results in a conformational change

challenge
unacceptableVIBCH:Q9KS122014-04-06 18:01:18 CDTGO:0009404 toxin metabolic process (P)PMID:19465933ECO:0000315 mutant phenotype evidence used in manual assertion

Figures 3,4 and 5 describe the conserved domain of the cysteine protease domain and show that it is actively catabolizing products. #5 shows the cysteine protease domain binding site in vitro

challenge

acceptable:0
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Shookjoh

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorDaniel.Koktan,
Team Just Name It Something
SINDV:POLNGO:0039690 - positive stranded viral RNA replication (P)PMID:19395054ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Shookjoh

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