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PMID:24490592
| Citation |
Hunter, JL, Severin, GB, Koestler, BJ and Waters, CM (2014) The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP. BMC Microbiol. 14:22 |
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| Abstract |
Diguanylate cyclases (DGCs) regulate biofilm formation and motility in bacteria by synthesizing the second messenger cyclic di-GMP (c-di-GMP) in response to environmental stimuli. DGC enzymatic activity is believed to be dependent on the presence of a GG(D/E)EF active site motif, however approximately 25% of known DGCs contain a degenerate active site. The Vibrio cholerae protein VCA0965 contains an AGDEF active site and is presumed to be an inactive DGC. |
| Links |
PubMed PMC3974145 Online version:10.1186/1471-2180-14-22 |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0052621: diguanylate cyclase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0052621: diguanylate cyclase activity |
ECO:0000314: |
F |
Figure 4 clearly demonstrates VCA0965's ability to synthesize c-di-GMP using Liquid Chromatography and Mass Spectrophotometry. Also see Fig 5. |
complete | ||||
See also
References
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