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PMID:24490592

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Citation

Hunter, JL, Severin, GB, Koestler, BJ and Waters, CM (2014) The Vibrio cholerae diguanylate cyclase VCA0965 has an AGDEF active site and synthesizes cyclic di-GMP. BMC Microbiol. 14:22

Abstract

Diguanylate cyclases (DGCs) regulate biofilm formation and motility in bacteria by synthesizing the second messenger cyclic di-GMP (c-di-GMP) in response to environmental stimuli. DGC enzymatic activity is believed to be dependent on the presence of a GG(D/E)EF active site motif, however approximately 25% of known DGCs contain a degenerate active site. The Vibrio cholerae protein VCA0965 contains an AGDEF active site and is presumed to be an inactive DGC.

Links

PubMed PMC3974145 Online version:10.1186/1471-2180-14-22

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

VIBCH:Q9KKY5

enables

GO:0052621: diguanylate cyclase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

VIBCH:Q9KKY5

GO:0052621: diguanylate cyclase activity

ECO:0000314:

F

Figure 4 clearly demonstrates VCA0965's ability to synthesize c-di-GMP using Liquid Chromatography and Mass Spectrophotometry. Also see Fig 5.

complete
CACAO 10048

See also

References

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