User:Schmitz4

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	ECOLI:RPOH	2013-09-29 06:41:05 CDT	GO:0033554 cellular response to stress (P)	PMID:1429436	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 4: Beta-galactosidase activity increased six times higher in htpR+ cells harboring TRZ plasmid (luxI,luxR, and lacZ). This demonstrates HtpR and its stimulation of LuxR protein synthesis	challenge
unacceptable	ECOLI:RPOH	2013-09-29 06:49:01 CDT	GO:0033554 cellular response to stress (P)	PMID:1429436	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 5 presents a typical strong induction of the lux system by added concentrations of a synthetic inducer from Vibrio fischeri. Early induction of the lux system by low concentrations of inducer occurs only when LuxR protein is present and active.	challenge
unacceptable	ECOLI:RPOH	2013-09-29 07:02:34 CDT	GO:0033554 cellular response to stress (P)	PMID:1429436	ECO:0000314 direct assay evidence used in manual assertion	figure 6 shows wild-type cells in stationary phase respond immediately to presence of inducer, whereas wild-type cells of other logarithmic growth phases do not.	challenge
unacceptable	ECOLI:RPOH	2013-09-29 07:02:34 CDT	GO:0033554 cellular response to stress (P)	PMID:1429436	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 7 shows that addition of LuxR protein advances luminescence induction of htpR+ cells but has no affect on htpR mutant cells.	challenge
unacceptable	ECOLI:RPOH	2013-09-29 07:07:11 CDT	GO:0033554 cellular response to stress (P)	PMID:1429436	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 8: transforming groESL-containing plasmids into htpR mutant cells showed luminescence induction at a much earlier growth stage than wild-type cells without the plasmid. Luciferase activity of htpR mutant cells was fully recovered by presence of GroESL proteins.	challenge

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unacceptable:5
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flagged:0

Annotations challenged by Schmitz4

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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