User:Santoro9

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	YEAST:MUC1	2014-04-06 09:59:23 CDT	GO:0044409 entry into host (P)	PMID:17921350	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1B showed the sigma1278b strain plated onto agar before and after washing. The WT could grow into the agar while the flo11 knockout could not	challenge
unacceptable	YEAST:MUC1	2014-04-06 09:59:24 CDT	GO:0000128 flocculation (P)	PMID:17921350	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1A: Flocculation assay tests whether or not the cells aggregate together and drop to the bottom of the tube. The yeast does flocculate in the WT as the cells collect at the bottom. The knockout stays cloudy in the entire solution for the whole time.	challenge
unacceptable	YEAST:MUC1	2014-04-06 09:59:24 CDT	GO:0043708 cell adhesion involved in biofilm formation (P)	PMID:17921350	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1D: Adherence assay. Growing strain on plastic tests biofilm formation capabilities. There is growth seen on the WT plate but not with the knockout.	challenge
unacceptable	YEAST:MUC1	2014-04-06 09:59:24 CDT	GO:0016337 cell-cell adhesion (P)	PMID:17921350	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 - Bead adhesion assay. Beads coated with the cells are placed on the plate to test cell-to-cell adhesion. In the WT, the growth is focused around the beads (black dots), while the growth in the knockout is not focused around the beads and is spread out around the plate.	challenge
unacceptable	YEAST:MUC1	2014-04-06 09:59:25 CDT	GO:0030447 response to nitrate starvation (P)	PMID:17921350	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1C: Pictures of yeast plated. FLO11 gene controls hyphae (filaments) formation. WT grows hyphae in order to find more nutrients, while the knockout does not.	challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Santoro9

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Gandii2, Team Protein Titans	?	GO:0035525 - NF-kappaB p50/p65 complex (Figure 2A-C shows that in cells exposed to arsentite, subunit p50 inhibits degradation of the C-Myc protein. In the absense of subunit p50, C-Myc protein levels were unstable.)	PMID:24457827	IDA: Inferred from Direct Assay	challenge	C: 2

0 annotations fixed by Santoro9

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